Batzenschlager Morgane, Schmit Anne-Catherine, Herzog Etienne, Fuchs Joerg, Schubert Veit, Houlné Guy, Chabouté Marie-Edith
a Institut de Biologie Moléculaire des Plantes, CNRS , Université de Strasbourg , Strasbourg , France.
b Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben , Stadt Seeland , Germany.
Nucleus. 2017 Jan 2;8(1):98-105. doi: 10.1080/19491034.2016.1276142.
The control of genomic maintenance during S phase is crucial in eukaryotes. It involves the establishment of sister chromatid cohesion, ensuring faithful chromosome segregation, as well as proper DNA replication and repair to preserve genetic information. In animals, nuclear periphery proteins - including inner nuclear membrane proteins and nuclear pore-associated components - are key factors which regulate DNA integrity. Corresponding functional homologues are not so well known in plants which may have developed specific mechanisms due to their sessile life. We have already characterized the Gamma-tubulin Complex Protein 3-interacting proteins (GIPs) as essential regulators of centromeric cohesion at the nuclear periphery. GIPs were also shown to interact with TSA1, first described as a partner of the epigenetic regulator MGOUN3 (MGO3)/BRUSHY1 (BRU1)/TONSOKU (TSK) involved in genomic maintenance. Here, using genetic analyses, we show that the mgo3gip1 mutants display an impaired and pleiotropic development including fasciation. We also provide evidence for the contribution of both MGO3 and GIP1 to the regulation of centromeric cohesion in Arabidopsis.
在真核生物中，S期基因组维持的控制至关重要。它涉及姐妹染色单体黏连的建立，以确保染色体的准确分离，以及适当的DNA复制和修复以保存遗传信息。在动物中，核周蛋白——包括内核膜蛋白和核孔相关成分——是调节DNA完整性的关键因素。在植物中，相应的功能同源物并不那么为人所知，由于其固着生活，植物可能已经发展出了特定的机制。我们已经将γ-微管蛋白复合体蛋白3相互作用蛋白（GIPs）鉴定为核周着丝粒黏连的重要调节因子。GIPs还被证明与TSA1相互作用，TSA1最初被描述为参与基因组维持的表观遗传调节因子MGOUN3（MGO3）/BRUSHY1（BRU1）/TONSOKU（TSK）的一个伙伴。在这里，通过遗传分析，我们表明mgo3gip1突变体表现出包括茎扁化在内的受损且多效的发育。我们还提供了证据，证明MGO3和GIP1都对拟南芥着丝粒黏连的调节有贡献。
