Bertrand J R, Rayner B, Imbach J L, Paoletti C, Malvy C
UA 147 CNRS, U 140 INSERM, Institut Gustave Roussy, Villejuif, France.
Biochem Biophys Res Commun. 1989 Oct 16;164(1):311-8. doi: 10.1016/0006-291x(89)91719-1.
Alpha-anomeric oligonucleotides are resistant to nucleases and display parallel annealing to RNA complementary sequences. We compared the effect of alpha- and beta-oligonucleotides targeted against various mRNA regions on the rabbit beta globin in vitro synthesis. In order to determine the role of RNase H, experiments were performed in both rabbit reticulocyte lysate and wheat germ extract. As expected beta-oligonucleotides were found more efficient in wheat germ extract which is rich in RNase H activity and alpha-oligonucleotide targeted against the initiation codon or downstream had no effect because they do not induce mRNA cleavage by RNase H. However, we report, for the first time, a specific translation inhibition by alpha-oligonucleotides. This occurs provided they are targeted against the cap region in 5' of the mRNA.
α-异头寡核苷酸对核酸酶具有抗性，并能与RNA互补序列进行平行退火。我们比较了针对兔β珠蛋白体外合成中不同mRNA区域的α-和β-寡核苷酸的作用。为了确定核糖核酸酶H（RNase H）的作用，在兔网织红细胞裂解物和小麦胚芽提取物中都进行了实验。正如预期的那样，在富含RNase H活性的小麦胚芽提取物中发现β-寡核苷酸更有效，而针对起始密码子或下游的α-寡核苷酸没有作用，因为它们不会诱导RNase H介导的mRNA切割。然而，我们首次报道了α-寡核苷酸的特异性翻译抑制作用。这种情况发生在它们靶向mRNA 5'端的帽区时。
