Cazenave C, Loreau N, Toulmé J J, Hélène C
Biochimie. 1986 Sep;68(9):1063-9. doi: 10.1016/s0300-9084(86)80180-8.
Oligodeoxyribonucleotides complementary to the initiation region of rabbit beta-globin messenger RNA were used to selectively inhibit translation in a wheat germ extract and in injected Xenopus oocytes. The oligonucleotides interacted specifically with their RNA target as shown by thermal denaturation studies of hybrids on nitrocellulose filters. The longest oligonucleotide used (17-mer) efficiently blocked translation both in vitro and in vivo. In contrast the shortest one (8-mer) exhibited only a limited effect. The translation block was specific. The synthesis of endogenous proteins in oocytes and that of alpha-globin in the in vitro system were not affected by anti-beta-globin oligonucleotides. A non-complementary oligonucleotide had no inhibitory effect.
与兔β-珠蛋白信使核糖核酸起始区域互补的寡脱氧核糖核苷酸被用于在小麦胚芽提取物和注射的非洲爪蟾卵母细胞中选择性抑制翻译。如通过硝酸纤维素滤膜上杂交体的热变性研究所示，这些寡核苷酸与其RNA靶标特异性相互作用。所使用的最长寡核苷酸（17聚体）在体外和体内均有效阻断翻译。相比之下，最短的一个（8聚体）仅表现出有限的作用。翻译阻断是特异性的。卵母细胞中内源性蛋白质的合成以及体外系统中α-珠蛋白的合成不受抗β-珠蛋白寡核苷酸的影响。非互补寡核苷酸没有抑制作用。
