Kyushiki H, Ikai A
Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, Japan.
Biochem Biophys Res Commun. 1989 Oct 16;164(1):434-8. doi: 10.1016/0006-291x(89)91738-5.
When fatty acid synthetase of the Harderian gland of guinea-pig was treated with various amounts of phenylmethanesulfonyl fluoride, the overall activity of the enzyme showed a quadratic decrease with respect to the inhibition degree of the thioesterase activity which was the primary target of inhibition. Moreover, the overall activity per active center of a heterodimer, which was formed between the native monomer and the thioesterase-less monomer, was higher than that of the native enzyme. These results are consistent with the view that the two active centers of the native enzyme exhibit a negative interference to each other.
用不同量的苯甲基磺酰氟处理豚鼠哈德氏腺脂肪酸合成酶时，该酶的总活性相对于作为主要抑制靶点的硫酯酶活性的抑制程度呈二次方下降。此外，天然单体与无硫酯酶单体之间形成的异二聚体每个活性中心的总活性高于天然酶。这些结果与天然酶的两个活性中心相互表现出负干扰的观点一致。
