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转酰基作用作为哺乳动物脂肪酸合成酶脂肪酸合成中的链终止机制。泌乳奶牛乳腺脂肪酸合成酶合成丁酸和己酸。

Transacylation as a chain-termination mechanism in fatty acid synthesis by mammalian fatty acid synthetase. Synthesis of butyrate and hexanoate by lactating cow mammary gland fatty acid synthetase.

作者信息

Hansen J K, Knudsen J

出版信息

Biochem J. 1980 Jan 15;186(1):287-94. doi: 10.1042/bj1860287.

DOI:10.1042/bj1860287
PMID:7370014
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1161529/
Abstract
  1. Purified cow mammary gland fatty acid synthetase synthesized long-chain unesterified and short-chain esterified fatty acids. 2. A direct relationship was observed between the amount of short-chain products synthesized and the concentration of acetyl-CoA in the incubation medium. 3. The short-chain products were identified as butyryl-CoA and hexanoyl-CoA. 4. Inhibition of the terminating thioester hydrolase of the fatty acid synthetase complex with phenylmethanesulphonyl fluoride did not inhibit the synthesis of short-chain products. 5. It is suggested that the synthesis of short-chain fatty acids involves the reverse of the 'loading' reaction.
摘要
  1. 纯化的牛乳腺脂肪酸合成酶能合成长链未酯化脂肪酸和短链酯化脂肪酸。2. 观察到合成的短链产物量与孵育培养基中乙酰辅酶A的浓度之间存在直接关系。3. 短链产物被鉴定为丁酰辅酶A和己酰辅酶A。4. 用苯甲磺酰氟抑制脂肪酸合成酶复合物的终止硫酯水解酶并不抑制短链产物的合成。5. 有人提出，短链脂肪酸的合成涉及“加载”反应的逆向过程。

相似文献

1
Transacylation as a chain-termination mechanism in fatty acid synthesis by mammalian fatty acid synthetase. Synthesis of butyrate and hexanoate by lactating cow mammary gland fatty acid synthetase.转酰基作用作为哺乳动物脂肪酸合成酶脂肪酸合成中的链终止机制。泌乳奶牛乳腺脂肪酸合成酶合成丁酸和己酸。
Biochem J. 1980 Jan 15;186(1):287-94. doi: 10.1042/bj1860287.
2
Transacylation as a chain-termination mechanism in fatty acid synthesis by mammalian fatty acid synthetase. Synthesis of medium-chain-length (C8-C12) acyl-CoA esters by goat mammary-gland fatty acid synthetase.转酰基作用作为哺乳动物脂肪酸合成酶催化脂肪酸合成过程中的链终止机制。山羊乳腺脂肪酸合成酶催化中链长度（C8 - C12）酰基辅酶A酯的合成。
Biochem J. 1982 Jan 15;202(1):139-43. doi: 10.1042/bj2020139.
3
Medium-chain fatty acid synthesis in lactating-rabbit mammary gland. Intracellular concentration and specificity of medium-chain acyl thioester hydrolase.泌乳兔乳腺中中链脂肪酸的合成。中链酰基硫酯水解酶的细胞内浓度及特异性。
Biochem J. 1979 Aug 1;181(2):267-74. doi: 10.1042/bj1810267.
4
Fatty-acid synthesis in lactating-goat mammary gland. 2. Medium-chain fatty acid synthesis.泌乳山羊乳腺中的脂肪酸合成。2. 中链脂肪酸合成。
Eur J Biochem. 1979 Apr;95(3):503-7. doi: 10.1111/j.1432-1033.1979.tb12990.x.
5
The interaction of fatty acid synthetase with cytoplasmic protein in the control of the chain-length of fatty acids synthesised by the lactating rabbit mammary gland.脂肪酸合成酶与细胞质蛋白在泌乳兔乳腺合成脂肪酸链长度控制中的相互作用。
Biochim Biophys Acta. 1976 Jan 18;486(1):91-102. doi: 10.1016/0005-2760(77)90073-x.
6
Fatty acid synthetase from lactating rat mammary gland. II. Studies on the termination sequence.来自泌乳大鼠乳腺的脂肪酸合成酶。II. 终止序列的研究。
J Biol Chem. 1971 Apr 25;246(8):2537-42.
7
Medium-chain fatty acid synthesis by goat mammary-gland fatty acid synthetase. The effect of limited proteolysis.山羊乳腺脂肪酸合成酶合成中链脂肪酸。有限蛋白酶解的影响。
Biochem J. 1983 Jan 1;209(1):215-22. doi: 10.1042/bj2090215.
8
Purification and some properties of a medium-chain acyl-thioester hydrolase from lactating-rabbit mammary gland which terminates chain elongation in fatty acid synthesis.来自泌乳兔乳腺的一种中链酰基硫酯水解酶的纯化及其某些特性，该酶可终止脂肪酸合成中的链延伸。
Biochem J. 1976 Dec 15;160(3):683-91. doi: 10.1042/bj1600683.
9
Fatty-acid synthesis in lactating-goat mammary gland. 1. Medium-chain fatty-acid synthesis.泌乳山羊乳腺中的脂肪酸合成。1. 中链脂肪酸合成。
Eur J Biochem. 1979 Apr;95(3):497-502. doi: 10.1111/j.1432-1033.1979.tb12989.x.
10
Acyl-CoA hydrolase(s) in rabbit mammary gland which control the chain length of fatty acids synthesised.兔乳腺中控制脂肪酸合成链长度的酰基辅酶A水解酶。
Biochem Biophys Res Commun. 1975 Aug 4;65(3):921-6. doi: 10.1016/s0006-291x(75)80473-6.

引用本文的文献

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How Close Are We to the Production of Milk in Alternative Systems? The Fat Perspective.我们距离在替代系统中生产牛奶还有多远？从脂肪角度来看。
Foods. 2025 Feb 26;14(5):809. doi: 10.3390/foods14050809.
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Distinctive Lipogenic Gene Expression Patterns in the Mammary Glands of Dairy Cows Are Associated with the Unique Fatty Acid Composition of Bovine Milk Fat.奶牛乳腺中独特的脂肪生成基因表达模式与牛乳脂肪独特的脂肪酸组成相关。
Foods. 2025 Jan 27;14(3):412. doi: 10.3390/foods14030412.
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MicroRNAs synergistically regulate milk fat synthesis in mammary gland epithelial cells of dairy goats.微小RNA协同调节奶山羊乳腺上皮细胞中的乳脂肪合成。
Gene Expr. 2013;16(1):1-13. doi: 10.3727/105221613x13776146743262.
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Triacylglycerol synthesis in goat mammary gland. Factors influencing the esterification of fatty acids synthesized de novo.山羊乳腺中的三酰甘油合成。影响从头合成脂肪酸酯化的因素。
Biochem J. 1984 Jun 1;220(2):521-7. doi: 10.1042/bj2200521.
5
Medium-chain fatty acid synthesis by goat mammary-gland fatty acid synthetase. The effect of limited proteolysis.山羊乳腺脂肪酸合成酶合成中链脂肪酸。有限蛋白酶解的影响。
Biochem J. 1983 Jan 1;209(1):215-22. doi: 10.1042/bj2090215.
6
Triacylglycerol synthesis in goat mammary gland. The effect of ATP, Mg2+ and glycerol 3-phosphate on the esterification of fatty acids synthesized de novo.山羊乳腺中三酰甘油的合成。ATP、Mg2+和3-磷酸甘油对从头合成的脂肪酸酯化作用的影响。
Biochem J. 1984 Jun 1;220(2):513-9. doi: 10.1042/bj2200513.
7
Evidence that the medium-chain acyltransferase of lactating-goat mammary-gland fatty acid synthetase is identical with the acetyl/malonyltransferase.泌乳山羊乳腺脂肪酸合成酶的中链酰基转移酶与乙酰/丙二酸单酰转移酶相同的证据。
Biochem J. 1985 May 1;227(3):981-5. doi: 10.1042/bj2270981.
8
Amino acid sequence around the active-site serine residue in the acyltransferase domain of goat mammary fatty acid synthetase.山羊乳腺脂肪酸合成酶酰基转移酶结构域中活性位点丝氨酸残基周围的氨基酸序列。
Biochem J. 1985 Apr 1;227(1):21-7. doi: 10.1042/bj2270021.

本文引用的文献

1
[On the biosynthesis of fatty acids. II. Synthesis and properties of S-malonyl-coenzyme A].[关于脂肪酸的生物合成。II. S-丙二酰辅酶A的合成与性质]
Biochem Z. 1962;335:540-7.
2
Fatty acid biosynthesis. VII. Substrate control of chain-length of products synthesised by rat liver fatty acid synthetase.脂肪酸生物合成。VII. 大鼠肝脏脂肪酸合成酶合成产物链长的底物控制。
Biochim Biophys Acta. 1970 Sep 8;210(3):400-10. doi: 10.1016/0005-2760(70)90035-4.
3
Fatty acid biosynthesis. V. Purification and characterisation of fatty acid synthetase from lactating-rabbit mammary gland.脂肪酸生物合成。V. 泌乳兔乳腺脂肪酸合成酶的纯化与特性鉴定。
Biochim Biophys Acta. 1970 Sep 8;210(3):371-87. doi: 10.1016/0005-2760(70)90033-0.
4
Fatty acid biosynthesis. VI. Specificity for termination of fatty acid biosynthesis by fatty acid synthetase from lactating-rabbit mammary gland.脂肪酸生物合成。VI. 泌乳兔乳腺脂肪酸合成酶终止脂肪酸生物合成的特异性。
Biochim Biophys Acta. 1970 Sep 8;210(3):388-99. doi: 10.1016/0005-2760(70)90034-2.
5
A convenient and versatile method for the purification of CoA thiol esters.一种用于纯化辅酶A硫酯的便捷通用方法。
Anal Biochem. 1973 Jul;54(1):188-98. doi: 10.1016/0003-2697(73)90262-5.
6
Fatty acid synthetase from cow mammary gland tissue cells.来自奶牛乳腺组织细胞的脂肪酸合成酶。
Biochim Biophys Acta. 1972 Nov 30;280(3):408-14. doi: 10.1016/0005-2760(72)90246-9.
7
Crotonyl coenzyme A reductase activity of bovine mammary fatty acid synthetase.牛乳腺脂肪酸合成酶的巴豆酰辅酶A还原酶活性
J Biol Chem. 1974 Jan 10;249(1):111-7.
8
The role of particulate esterification in chain length control of de novo synthesized fatty acids in liver and mammary gland.微粒体酯化在肝脏和乳腺中从头合成脂肪酸链长控制中的作用。
Comp Biochem Physiol B. 1976;53(1):3-7. doi: 10.1016/0305-0491(76)90086-9.
9
Acyl-CoA hydrolase(s) in rabbit mammary gland which control the chain length of fatty acids synthesised.兔乳腺中控制脂肪酸合成链长度的酰基辅酶A水解酶。
Biochem Biophys Res Commun. 1975 Aug 4;65(3):921-6. doi: 10.1016/s0006-291x(75)80473-6.
10
Characterization of fatty acid synthesis by cow mammary subcellular fractions.奶牛乳腺亚细胞组分脂肪酸合成的特性分析
J Dairy Sci. 1976 Aug;59(8):1513-7. doi: 10.3168/jds.S0022-0302(76)84394-9.