Almeida Fernanda, Medeiros Marta Inês Cazentini, Rodrigues Dália Dos Prazeres, Allard Marc W, Falcão Juliana Pfrimer
Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Av. do Café s/n°, Ribeirão Preto, SP, Brazil.
Centro de Laboratório Regional de Ribeirão Preto, Instituto Adolfo Lutz, Rua Minas 877, Ribeirão Preto, SP, Brazil.
J Microbiol Methods. 2017 Feb;133:55-61. doi: 10.1016/j.mimet.2016.12.020. Epub 2016 Dec 26.
CRISPR-multi-locus virulence sequence typing (CRISPR-MVLST) was performed to type 92 S. Typhimurium strains isolated from humans and food sources between 1983 and 2013 in Brazil and assess the suitability of this methodology comparing it with PFGE already used for subtyping the same strains. Among the 92 S. Typhimurium strains studied, we identified 25 CRISPR1 alleles, 27 CRISPR2 alleles, 2 fimH alleles and 3 sseL alleles showing that the genetic variability is much higher in the CRISPRs loci than in the virulence genes. The CRISPR-MVLST analysis provided similar results to the PFGE previously published used to type the same set of strains, demonstrating that CRISPR-MVLST is a very efficient approach for subtyping S. Typhimurium serovar and can be used to complement and validate results obtained by the PFGE methodology.
进行了CRISPR多位点毒力序列分型（CRISPR-MVLST），以对1983年至2013年间在巴西从人类和食物来源分离出的92株鼠伤寒沙门氏菌菌株进行分型，并通过与已用于对相同菌株进行亚型分型的PFGE方法比较，评估该方法的适用性。在所研究的92株鼠伤寒沙门氏菌菌株中，我们鉴定出25个CRISPR1等位基因、27个CRISPR2等位基因、2个fimH等位基因和3个sseL等位基因，表明CRISPR位点的遗传变异性远高于毒力基因。CRISPR-MVLST分析提供了与先前发表的用于对同一组菌株进行分型的PFGE相似的结果，表明CRISPR-MVLST是一种对鼠伤寒沙门氏菌血清型进行亚型分型的非常有效的方法，可用于补充和验证通过PFGE方法获得的结果。
