Department of Population Health, College of Veterinary Medicine, University of Georgia, Athens, GA, USA.
Department of Food Science, College of Agricultural Sciences, The Pennsylvania State University, University Park, PA, USA.
Methods Mol Biol. 2021;2182:39-44. doi: 10.1007/978-1-0716-0791-6_5.
Polymerase chain reaction (PCR) and sequencing-based subtyping tools are useful for rapid analyses of Salmonella isolates. Here we describe the process of clustered regularly interspaced short palindromic repeat-multiple virulence locus sequence typing (CRISPR-MVLST) for Salmonella subtyping.
聚合酶链式反应(PCR)和基于测序的分型工具对于沙门氏菌分离株的快速分析非常有用。本文描述了用于沙门氏菌分型的聚类规则间隔短回文重复序列-多位点可变数目串联重复序列分型(CRISPR-MVLST)的过程。