Chen Jicheng, Tian Jingjing, Ge Huifang, Liu Ruihai, Xiao Jianbo
College of Food Science, Fujian Agriculture and Forest University, 15 Shangxiadian Rd, Fuzhou, 350002, PR China; Department of Food Science, Cornell University, Ithaca, NY, 14853, USA.
College of Food Science, Fujian Agriculture and Forest University, 15 Shangxiadian Rd, Fuzhou, 350002, PR China.
Food Chem Toxicol. 2017 Nov;109(Pt 2):930-940. doi: 10.1016/j.fct.2016.12.017. Epub 2016 Dec 26.
Tetramethylpyrazine is a bioactive compound found in Chinese black vinegar. This work is to investigate the potential effects of tetramethylpyrazine on intracellular cholesterol modulation in HepG2 cells. The results demonstrated that tetramethylpyrazine can induce intracellular cholesterol efflux. Tetramethylpyrazine may also improve endothelial function through its antioxidant effects by inhibiting reactive oxygen species levels and increasing the antioxidant enzymes superoxide dismutase and catalase. Tetramethylpyrazine increased liver X receptor and peroxisome proliferator-activated receptor gene expression in HepG2 cells. Protein expression of ATP-binding cassette transporter 1 was up-regulated in a dose-dependent manner (P < 0.05). The biological significance of tetramethylpyrazine may involve hypolipidemic effects via modulation of intracellular cholesterol efflux, ROS inhibition, increases in SOD and CAT activities, and direct regulation of PPAR and LXR gene expression. Tetramethylpyrazine may improve lipid profiles by elevating the PPARγ-LXRα-ABCA1 pathway.
川芎嗪是一种存在于中国黑醋中的生物活性化合物。这项工作旨在研究川芎嗪对HepG2细胞内胆固醇调节的潜在影响。结果表明，川芎嗪可诱导细胞内胆固醇外流。川芎嗪还可能通过抑制活性氧水平并增加抗氧化酶超氧化物歧化酶和过氧化氢酶，发挥其抗氧化作用来改善内皮功能。川芎嗪增加了HepG2细胞中肝脏X受体和过氧化物酶体增殖物激活受体基因的表达。ATP结合盒转运蛋白1的蛋白表达呈剂量依赖性上调（P<0.05）。川芎嗪的生物学意义可能涉及通过调节细胞内胆固醇外流、抑制ROS、增加SOD和CAT活性以及直接调节PPAR和LXR基因表达来发挥降血脂作用。川芎嗪可能通过提高PPARγ-LXRα-ABCA1途径来改善血脂谱。
