Qi Z H, Yan J, Xiong W J, Yuan J G, Song S, Xue C Q, Cai L W
Department of Biochemistry, Chinese Academy of Medical Sciences, Beijing.
Sci China B. 1989 Sep;32(9):1082-6.
On the basis of sequencing the large DNA-fragments which have been inserted into M13mp8, we design a simple strategy to determine the complete nucleotide sequence of HBV adr NC-1 DNA with chain termination method. The whole genome is 3195 nucleotides long. Five reading frames are observed. The gene location and organization are shown.
在对插入到M13mp8中的大DNA片段进行测序的基础上,我们设计了一种简单的策略,用链终止法测定乙肝病毒adr NC-1 DNA的完整核苷酸序列。整个基因组长度为3195个核苷酸。观察到五个阅读框。显示了基因定位和组织情况。