Seo Sojin, Kim Daeyoung, Song Wooseok, Heo Jihune, Joo Minju, Lim Yeri, Yeom Ji-Hyun, Lee Kangseok
Department of Life Science, Chung-Ang University, Seoul, 06974, Republic of Korea.
J Microbiol. 2017 Jan;55(1):37-43. doi: 10.1007/s12275-017-6518-0. Epub 2016 Dec 30.
RraA is a protein inhibitor of RNase E, which degrades and processes numerous RNAs in Escherichia coli. Streptomyces coelicolor also contains homologs of RNase E and RraA, RNase ES and RraAS1/RraAS2, respectively. Here, we report that, unlike other RraA homologs, RraAS1 directly interacts with the catalytic domain of RNase ES to exert its inhibitory effect. We further show that rraAS1 gene deletion in S. coelicolor results in a higher growth rate and increased production of actinorhodin and undecylprodigiosin, compared with the wild-type strain, suggesting that RraAS1-mediated regulation of RNase ES activity contributes to modulating the cellular physiology of S. coelicolor.
RraA是核糖核酸酶E的一种蛋白质抑制剂，核糖核酸酶E可降解和加工大肠杆菌中的多种RNA。天蓝色链霉菌也分别含有核糖核酸酶E和RraA的同源物，即核糖核酸酶ES和RraAS1/RraAS2。在此，我们报告，与其他RraA同源物不同，RraAS1直接与核糖核酸酶ES的催化结构域相互作用以发挥其抑制作用。我们进一步表明，与野生型菌株相比，天蓝色链霉菌中rraAS1基因缺失导致更高的生长速率以及放线紫红素和十一烷基灵菌红素产量增加，这表明RraAS1介导的核糖核酸酶ES活性调节有助于调节天蓝色链霉菌的细胞生理。
