Han Guangda, Wang Jin-Tao, Ji Xiaotian, Liu Li, Zhao Hanying
Key Laboratory of Functional Polymer Materials, Ministry of Education, College of Chemistry, Nankai University; Collaborative Innovation Center of Chemical Science and Engineering (Tianjin) , Tianjin 300071, China.
Bioconjug Chem. 2017 Feb 15;28(2):636-641. doi: 10.1021/acs.bioconjchem.6b00704. Epub 2017 Jan 10.
Proteinosomes are a type of protein-based spherical capsules, which have potential applications in drug delivery, cell imaging, gene expression, and biocatalysis. In this research, a novel approach to the fabrication of proteinosomes entirely composed of protein molecules based on self-assembly of a supramolecular protein-polymer conjugate is proposed. A supramolecular protein-polymer conjugate was prepared by mixing βCD-modified bovine serum albumin (BSA) and adamantane-terminated poly(N-isopropylamide) (Ad-PNIPAM) in aqueous solution. The BSA-PNIPAM bioconjugate self-assembled into micelles with PNIPAM cores and BSA coronae at a temperature above the lower critical solution temperature (LCST) of PNIPAM. After cross-linking of BSA in the coronae, and followed by addition of excess βCD, PNIPAM chains were cleaved from the micellar structures, and nanoscale proteinosomes were prepared. The dual-responsive proteinosomes dissociated in the presence of trypsin or glutathione.
蛋白质体是一种基于蛋白质的球形胶囊，在药物递送、细胞成像、基因表达和生物催化方面具有潜在应用。在本研究中，提出了一种基于超分子蛋白质-聚合物共轭物自组装制备完全由蛋白质分子组成的蛋白质体的新方法。通过在水溶液中混合β-环糊精修饰的牛血清白蛋白（BSA）和金刚烷封端的聚（N-异丙基丙烯酰胺）（Ad-PNIPAM）制备了超分子蛋白质-聚合物共轭物。在高于PNIPAM的低临界溶液温度（LCST）的温度下，BSA-PNIPAM生物共轭物自组装成具有PNIPAM核和BSA冠层的胶束。在冠层中的BSA交联后，接着加入过量的β-环糊精，PNIPAM链从胶束结构中裂解，制备出纳米级蛋白质体。这种双响应蛋白质体在胰蛋白酶或谷胱甘肽存在下会解离。
