Qin Yan, Yi Jianguo, Zhang Yue
Hebei Key Laboratory of Functional Polymers, School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin 300130, China.
Nanomaterials (Basel). 2023 May 25;13(11):1725. doi: 10.3390/nano13111725.
In recent years, the coupling of structurally and functionally controllable polymers with biologically active peptide materials to obtain polymer-peptide hybrids with excellent properties and biocompatibility has led to important research progress in the field of polymers. In this study, a pH-responsive hyperbranched polymer hPDPA was prepared by combining atom transfer radical polymerization (ATRP) with self-condensation vinyl polymerization (SCVP) using a three-component reaction of Passerini to obtain a monomeric initiator ABMA containing functional groups. The pH-responsive polymer peptide hybrids hPDPA/PArg/HA were obtained by using the molecular recognition of polyarginine (β-CD-PArg) peptide modified with β-cyclodextrin (β-CD) on the hyperbranched polymer, followed by the electrostatic adsorption of hyaluronic acid (HA). The two hybrid materials, hPDPA/PArg/HA and hPDPA/PArg/HA could self-assemble to form vesicles with narrow dispersion and nanoscale dimensions in phosphate-buffered (PB) at pH = 7.4. The assemblies exhibited low toxicity as drug carriers of β-lapachone (β-lapa), and the synergistic therapy based on ROS and NO generated by β-lapa had significant inhibitory effects on cancer cells.
近年来，将结构和功能可控的聚合物与生物活性肽材料偶联，以获得具有优异性能和生物相容性的聚合物-肽杂化材料，这在聚合物领域取得了重要的研究进展。在本研究中，通过原子转移自由基聚合（ATRP）与自缩合乙烯基聚合（SCVP）相结合，利用Passerini三组分反应制备了一种pH响应性超支化聚合物hPDPA，以获得含官能团的单体引发剂ABMA。通过用β-环糊精（β-CD）修饰的聚精氨酸（β-CD-PArg）肽在超支化聚合物上的分子识别，随后进行透明质酸（HA）的静电吸附，获得了pH响应性聚合物肽杂化材料hPDPA/PArg/HA。这两种杂化材料hPDPA/PArg/HA和hPDPA/PArg/HA在pH = 7.4的磷酸盐缓冲液（PB）中可自组装形成具有窄分散性和纳米尺寸的囊泡。这些组装体作为β-拉帕醌（β-lapa）的药物载体表现出低毒性，并且基于β-lapa产生的活性氧（ROS）和一氧化氮（NO）的协同治疗对癌细胞具有显著的抑制作用。
