Yoo SooYeon, Ahn Hyejin, Park Yoo Kyoung
Department of Medical Nutrition, Kyung Hee University, 1732 Deogyeong-daero, Giheung-gu, Yongin 17104, Korea.
Research Institute of Medical Nutrition, Kyung Hee University, 26, Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Korea.
Nutrients. 2016 Dec 26;9(1):11. doi: 10.3390/nu9010011.
The objective of this study was to determine the effects of a high-fructose diet on cardiovascular disease (CVD)-related parameters in growing rats. Three-week-old female Sprague Dawley rats were randomly assigned to four experimental groups; a regular diet group (RD: fed regular diet based on AIN-93G, = 8), a high-fructose diet group (30Frc: fed regular diet with 30% fructose, = 8), a high-fat diet group (45Fat: fed regular diet with 45 kcal% fat, = 8) or a high fructose with high-fat diet group (30Frc + 45Fat, fed diet 30% fructose with 45 kcal% fat, = 8). After an eight-week treatment period, the body weight, total-fat weight, serum glucose, insulin, lipid profiles and pro-inflammatory cytokines, abdominal aortic wall thickness, and expressions of eNOS and ET-1 mRNA were analyzed. The result showed that total-fat weight was higher in the 30Frc, 45Fat, and 30Frc + 45Fat groups compared to the RD group ( < 0.05). Serum triglyceride (TG) levels were highest in the 30Frc group than the other groups ( < 0.05). The abdominal aorta of 30Frc, 45Fat, and 30Frc + 45Fat groups had higher wall thickness than the RD group ( < 0.05). Abdominal aortic eNOS mRNA level was decreased in 30Frc, 45Fat, and 30Frc + 45Fat groups compared to the RD group ( < 0.05), and also 45Fat and 30Frc + 45Fat groups had decreased mRNA expression of eNOS compared to the 30Frc group ( < 0.05). ET-1 mRNA level was higher in 30Frc, 45Fat, and 30Frc + 45Fat groups than the RD group ( < 0.05). Both high fructose consumption and high fat consumption in growing rats had similar negative effects on CVD-related parameters.
本研究的目的是确定高果糖饮食对生长中大鼠心血管疾病（CVD）相关参数的影响。将三周龄雌性斯普拉格-道利大鼠随机分为四个实验组；正常饮食组（RD：基于AIN-93G喂养正常饮食，n = 8）、高果糖饮食组（30Frc：用30%果糖喂养正常饮食，n = 8）、高脂肪饮食组（45Fat：用45千卡%脂肪喂养正常饮食，n = 8）或高果糖高脂肪饮食组（30Frc + 45Fat，用30%果糖和45千卡%脂肪喂养饮食，n = 8）。经过八周的治疗期后，分析体重、总脂肪重量、血清葡萄糖、胰岛素、血脂谱和促炎细胞因子、腹主动脉壁厚度以及eNOS和ET-1 mRNA的表达。结果显示，与RD组相比，30Frc、45Fat和30Frc + 45Fat组的总脂肪重量更高（P < 0.05）。30Frc组的血清甘油三酯（TG）水平高于其他组（P < 0.05）。30Frc、45Fat和30Frc + 45Fat组的腹主动脉壁厚度高于RD组（P < 0.05）。与RD组相比，30Frc、45Fat和30Frc + 45Fat组腹主动脉eNOS mRNA水平降低（P < 0.05），并且与30Frc组相比，45Fat和30Frc + 45Fat组eNOS的mRNA表达也降低（P < 0.05）。30Frc、45Fat和30Frc + 45Fat组的ET-1 mRNA水平高于RD组（P < 0.05）。生长中大鼠食用高果糖和高脂肪对CVD相关参数具有相似的负面影响。
