Chaiyasut Chaiyavat, Sivamaruthi Bhagavathi Sundaram, Pengkumsri Noppawat, Keapai Waranya, Kesika Periyanaina, Saelee Manee, Tojing Parichart, Sirilun Sasithorn, Chaiyasut Khontaros, Peerajan Sartjin, Lailerd Narissara
Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand.
Department of Physiology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
Pharmaceuticals (Basel). 2016 Dec 28;10(1):3. doi: 10.3390/ph10010003.
: Diabetes mellitus (DM), particularly type 2 DM (T2DM), is one of the most common metabolic disorder worldwide. The prevention measures and treatment strategies for DM are improving steadily. The current study explains the impact of germination on phytochemical content of Thai black rice (BR), and the influence of germinated BR extract (GBRE) supplementation on diabetic conditions in rats. : BR was germinated and the phenolic, anthocyanin, and γ-aminobutyric acid (GABA) content of the extract were analyzed using HPLC and spectrophotometric methods. Streptozotocin-induced diabetic rats were supplemented with high and low doses of GBRE. The plasma glucose, insulin, cholesterol, triglyceride levels, antioxidant status, and antioxidant enzyme levels of treated animals were assessed using ELISA and spectrophotometric methods. : Germination enhanced the GABA content of BR, and GBRE intervention improved the total antioxidant capacity and antioxidant enzymes levels in diabetic rats. The plasma glucose, cholesterol, triglyceride levels, insulin resistance and glucose tolerance were reduced, and the degree of insulin secretion in rat plasma was significantly increased upon GBRE treatment. Both pre and post-treatment approaches showed the anti-diabetic ability of GBRE. In most of the analyzed parameters, GBRE was quite equal to the performance of drug-metformin. : GBRE supplementation helps prevent and manage the consequences of DM.
糖尿病（DM），尤其是2型糖尿病（T2DM），是全球最常见的代谢紊乱疾病之一。糖尿病的预防措施和治疗策略正在稳步改善。当前的研究解释了发芽对泰国黑米（BR）植物化学成分的影响，以及发芽黑米提取物（GBRE）补充剂对大鼠糖尿病状况的影响。：将黑米发芽，使用高效液相色谱法（HPLC）和分光光度法分析提取物中的酚类、花青素和γ-氨基丁酸（GABA）含量。用链脲佐菌素诱导糖尿病大鼠，并给予高剂量和低剂量的GBRE。使用酶联免疫吸附测定法（ELISA）和分光光度法评估受试动物的血糖、胰岛素、胆固醇、甘油三酯水平、抗氧化状态和抗氧化酶水平。：发芽提高了黑米的GABA含量，GBRE干预提高了糖尿病大鼠的总抗氧化能力和抗氧化酶水平。GBRE治疗后，大鼠的血糖、胆固醇、甘油三酯水平、胰岛素抵抗和糖耐量降低，大鼠血浆中胰岛素分泌程度显著增加。治疗前和治疗后的方法均显示GBRE具有抗糖尿病能力。在大多数分析参数中，GBRE的表现与药物二甲双胍相当。：补充GBRE有助于预防和控制糖尿病后果。
