Yoo Jae-Myung, Yang Ju-Hye, Kim Young Soo, Yang Hye Jin, Cho Won-Kyung, Ma Jin Yeul
Korean Medicine (KM)-Application Center, Korea Institute of Oriental Medicine (KIOM), 70 Cheomdan-ro, Dong-gu, Daegu 41062, Korea.
Molecules. 2016 Dec 28;22(1):37. doi: 10.3390/molecules22010037.
The accumulation and infiltration of mast cells are found in osteoarthritic lesions in humans and rodents. Nonetheless, the roles of mast cells in osteoarthritis are almost unknown. Although has various beneficial actions, its effect on allergic and osteoarthritic responses is unknown. In this study, we established an in vitro model of mast cell-mediated osteoarthritis and investigated the effect of the ethanol extract of (VEE) on IgE/antigen (IgE/Ag)-activated mast cells and mast cell-derived inflammatory mediator (MDIM)-stimulated chondrocytes. The anti-allergic effect of VEE was evaluated by degranulation, inflammatory mediators, and the FcεRI signaling cascade in IgE/Ag-activated RBL-2H3 cells. The anti-osteoarthritic action of VEE was evaluated by cell migration, and the expression, secretion, and activity of MMPs in MDIM-stimulated SW1353 cells. VEE significantly inhibited degranulation (IC: 93.04 μg/mL), the production of IL-4 (IC: 73.28 μg/mL), TNF-α (IC: 50.59 μg/mL), PGD₂ and LTC₄, and activation of the FcεRI signaling cascade in IgE/Ag-activated RBL-2H3 cells. Moreover, VEE not only reduced cell migration but also inhibited the expression, secretion, and/or activity of MMP-1, MMP-3, or MMP-13 in MDIM-stimulated SW1353 cells. In conclusion, VEE possesses both anti-allergic and anti-osteoarthritic properties. Therefore, VEE could possibly be considered a new herbal drug for anti-allergic and anti-osteoarthritic therapy. Moreover, the in vitro model may be useful for the development of anti-osteoarthritic drugs.
在人类和啮齿动物的骨关节炎病变中发现了肥大细胞的积累和浸润。尽管如此，肥大细胞在骨关节炎中的作用几乎仍不为人知。虽然[某物质]具有多种有益作用，但其对过敏和骨关节炎反应的影响尚不清楚。在本研究中，我们建立了肥大细胞介导的骨关节炎体外模型，并研究了[某植物名称]乙醇提取物（VEE）对IgE/抗原（IgE/Ag）激活的肥大细胞和肥大细胞衍生的炎症介质（MDIM）刺激的软骨细胞的影响。通过脱颗粒、炎症介质以及IgE/Ag激活的RBL-2H3细胞中的FcεRI信号级联反应来评估VEE的抗过敏作用。通过细胞迁移以及MDIM刺激的SW1353细胞中基质金属蛋白酶（MMPs）的表达、分泌和活性来评估VEE的抗骨关节炎作用。VEE显著抑制了IgE/Ag激活的RBL-2H3细胞中的脱颗粒（IC：93.04μg/mL）、IL-4（IC：73.28μg/mL）、TNF-α（IC：50.59μg/mL）、PGD₂和LTC₄的产生以及FcεRI信号级联反应的激活。此外，VEE不仅减少了细胞迁移，还抑制了MDIM刺激的SW1353细胞中MMP-1、MMP-3或MMP-13的表达、分泌和/或活性。总之，VEE具有抗过敏和抗骨关节炎特性。因此，VEE可能被认为是一种用于抗过敏和抗骨关节炎治疗的新型草药药物。此外，该体外模型可能有助于抗骨关节炎药物的开发。
