Research Institute of Pharmaceutical Sciences, College of Pharmacy, Kyungpook National University, 80 Daehak-ro, Daegu 41566, Republic of Korea.
Food Funct. 2016 May 18;7(5):2270-7. doi: 10.1039/c6fo00138f. Epub 2016 Apr 13.
Viscum album var. coloratum (Korean mistletoe; KM) is an herbal medicine that is used worldwide for the treatment of various immunological disorders and cancers. KM extract showed enhanced anti-oxidative effects in 2,2-diphenyl-1-picrylhydrazyl, Trolox equivalent antioxidant capacity, and 5-(and-6)-chloromethyl-2',7'-dichlorodihydrofluorescein diacetate acetyl ester assays after being fermented with a crude enzyme extract from a soybean paste fungus, Aspergillus kawachii. High-performance liquid chromatography analysis showed four increased peaks in enzyme treated KM. The increased peaks were isolated and identified as caffeic acid (1), hesperetin (2), syringaldehyde (3), and lyoniresinol (4). Among the four compounds, only 1 and 4 showed strong anti-oxidative activity. Therefore, the fermentation increased the contents of 1 and 4, which consequently increased the anti-oxidative activity of KM.
槲寄生（KM）是一种草药，在全球范围内被用于治疗各种免疫性疾病和癌症。用大豆曲霉菌 Aspergillus kawachii 的粗酶提取物发酵后，KM 提取物在 2,2-二苯基-1-苦基肼、Trolox 等效抗氧化能力和 5-(和-6)-氯甲基-2',7'-二氯二氢荧光素二乙酸乙酰酯乙酰酯测定中显示出增强的抗氧化效果。高效液相色谱分析显示，酶处理后的 KM 有四个增加的峰。增加的峰被分离并鉴定为咖啡酸（1）、橙皮素（2）、丁香醛（3）和丁香脂素（4）。在这四种化合物中，只有 1 和 4 表现出很强的抗氧化活性。因此，发酵增加了 1 和 4 的含量，从而提高了 KM 的抗氧化活性。
