Li Min, Cao Yu-Ting, Ye Si-Rui, Irshad Muhammad, Pan Teng-Fei, Qiu Dong-Liang
College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Molecules. 2016 Dec 29;22(1):44. doi: 10.3390/molecules22010044.
Chalcone synthase gene () from flowers was isolated using RT-PCR and RACE. The coding region of the gene is 1425-bp with an open reading frame of 1170-bp, 73-bp 5'UTR, and 172-bp 3'UTR. Its deduced protein does not have a signal peptide but does contain a cond_enzyme superfamily domain, and consists of 389 amino acids with a predicted molecular mass of 42,699 Da and a pI of 6.57. The deduced amino acid sequence of BaCHS shares 90%, 88%, 85%, 84% and 79% identity with CHS from , , , and , respectively. The striking color change from dark purple to light purple and ultimately lead to pure white resulted from a decline in anthocyanin content of the petals and was preceded by a decrease in the expression of . Its gene expression was positively correlated with the contents of anthocyanin ( ≤ 0.01).
利用RT-PCR和RACE技术从花中分离出查尔酮合酶基因（）。该基因的编码区为1425bp，开放阅读框为1170bp，5'UTR为73bp，3'UTR为172bp。其推导的蛋白质没有信号肽，但确实包含一个cond_enzyme超家族结构域，由389个氨基酸组成，预测分子量为42,699Da，pI为6.57。BaCHS推导的氨基酸序列与来自、、、和的CHS分别具有90%、88%、85%、84%和79%的同一性。从深紫色到浅紫色最终变为纯白色的显著颜色变化是由于花瓣中花青素含量的下降导致的，并且在此之前的表达量也有所下降。其基因表达与花青素含量呈正相关（≤0.01）。
