College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Department of Horticulture, Sylhet Agricultural University, Sylhet 3100, Bangladesh.
Genes (Basel). 2021 Jul 18;12(7):1086. doi: 10.3390/genes12071086.
The full-length cDNA sequence of gene from the was obtained by RT-PCR and RACE, whose GenBank accession number is JQ678765. The sequence contains a 1521 bp open reading frame, 120 bp 5'UTR and 61 bp 3'UTR, encoding a total of 506 amino acids. The molecular mass of the predicted protein is 56.47 kDa with an estimated pI of 8.78, respectively. Sequence alignment showed that the amino acid sequence of F3'5'H was 91%, 87% and 84% with that of , ., , respectively. Real-time quantitative PCR analysis showed that the expression of gene was different in petals of different days, which was the highest expression level on day 0 and significantly higher than other days. The results indicated that might play key role in flower color regulation and provide a theoretical reference for blue flower molecular breeding.
通过 RT-PCR 和 RACE 从 中获得了 基因的全长 cDNA 序列，其 GenBank 登录号为 JQ678765。该序列包含一个 1521 bp 的开放阅读框、120 bp 的 5'UTR 和 61 bp 的 3'UTR，编码总共 506 个氨基酸。预测蛋白的分子量为 56.47 kDa，等电点估计为 8.78。序列比对表明，F3'5'H 的氨基酸序列与 、.、.分别有 91%、87%和 84%的同源性。实时定量 PCR 分析表明，不同天数花瓣中 基因的表达存在差异，在第 0 天表达水平最高，明显高于其他天数。结果表明， 可能在花色调控中发挥关键作用，为蓝色花的分子育种提供了理论参考。
