Bodnar Giovana Carolina, Martins Heloísa Moreira, De Oliveira Caio Ferreira, Morey Alexandre Tadachi, Tavares Eliandro Reis, Cardoso Juscélio Donizete, Perugini Marcia Regina Eches, Lioni Lucy Megumi Yamauchi, Yamada-Ogatta Sueli Fumie, Kobayashi Renata Katsuko Takayama, Nakazato Gerson
Biological Sciences Center, Universidade Estadual de Londrina, Brazil.
J Infect Dev Ctries. 2016 Dec 30;10(12):1306-1317. doi: 10.3855/jidc.7887.
Infections caused by multidrug-resistant bacteria are increasingly common and represent a serious problem for public health. Staphylococcus aureus is one of the major agents of infections, and methicillin-resistant S. aureus (MRSA) has spread worldwide. The aim of this study was to phenotypically and genotypically characterize 55 MRSAs isolated in the University Hospital of Londrina, Paraná, Brazil, during 2010.
Bacterial isolates were characterized based on their antimicrobial susceptibility profile, biofilm production capacity, and staphylococcal chromosome cassette mec (SCCmec) type. Determination of clonal groups was performed by polymerase chain reaction using the RW3A, JB1, and BOX A1R primers and high-resolution melting (HRM) analysis.
The majority of isolates harbored SCCmec type II. SCCmec III, characteristic of the Brazilian endemic clone, was observed in four strains. Only two isolates harbored SCCmec type IV, which is common in community-acquired MRSA strains. Most isolates also showed resistance to more than four of the tested antimicrobials, and 30 isolates exhibited the ability to produce biofilm. DNA polymorphism analysis showed a higher discriminatory power for the JB1 primer, but RW3A revealed several clonal groups of MRSA with similar genotypic and phenotypic characteristics. HRM analysis showed eight different sequence types.
These results are important for epidemiological studies involving MRSA infections.
耐多药细菌引起的感染日益普遍，对公共卫生构成严重问题。金黄色葡萄球菌是主要的感染病原体之一，耐甲氧西林金黄色葡萄球菌（MRSA）已在全球传播。本研究的目的是对2010年期间在巴西巴拉那州隆德里纳大学医院分离出的55株MRSA进行表型和基因型特征分析。
根据细菌分离株的抗菌药物敏感性谱、生物膜产生能力和葡萄球菌染色体盒式mec（SCCmec）类型对其进行特征分析。使用RW3A、JB1和BOX A1R引物通过聚合酶链反应和高分辨率熔解（HRM）分析来确定克隆群。
大多数分离株携带II型SCCmec。在四株菌株中观察到巴西地方流行克隆特有的III型SCCmec。只有两株分离株携带IV型SCCmec，这在社区获得性MRSA菌株中很常见。大多数分离株还对四种以上的测试抗菌药物耐药，30株分离株具有产生生物膜的能力。DNA多态性分析显示JB1引物具有更高的鉴别力，但RW3A揭示了几个具有相似基因型和表型特征的MRSA克隆群。HRM分析显示了八种不同的序列类型。
这些结果对于涉及MRSA感染的流行病学研究很重要。
