Department of Microbiology, Center of Biological Sciences, Universidade Estadual de Londrina, Londrina, Paraná, Brazil.
NanoBioss, Institute of Chemistry, Universidade Estadual de Campinas, Campinas, São Paulo, Brazil.
Int J Nanomedicine. 2019 Oct 3;14:7975-7985. doi: 10.2147/IJN.S211756. eCollection 2019.
Multidrug-resistant bacteria such as extended-spectrum beta-lactamase (ESBL), Enterobacteriaceae, and methicillin-resistant (MRSA) pose a challenge to the human health care system. MRSA is among the major causes of hospital-acquired and community infections.
Therefore, in the present study, we evaluated the antibacterial activity of silver nanoparticles synthesized by (AgNP) in combination with simvastatin against reference and multidrug-resistant bacterial strains.
Simvastatin showed a minimal inhibitory concentration (MIC) ranging from 0.062 to 0.25 mg mL against MRSA. AgNP with a size of 77.68± 33.95 nm and zeta potential -34.6 ± 12.7 mV showed an MIC of 0.212 mg mL against including MRSA strains. The checkerboard assay and time-kill curves exhibited a synergistic effect of the simvastatin-AgNP combination on antibacterial activity against MRSA strains. The combination of simvastatin and AgNP demonstrated antibacterial activity against producing ESBL. Scanning electron microscopy showed the formation of cell surface protrusions after treatment with AgNP and the formation of a large amorphous mass after treatment with simvastatin, both in MRSA.
Our results indicate that the combination of AgNP and simvastatin could be a great future alternative in the control of bacterial infections, where, when combined with simvastatin, smaller doses of AgNP are required, with the same antibacterial activity.
产超广谱β-内酰胺酶（ESBL）、肠杆菌科和耐甲氧西林金黄色葡萄球菌（MRSA）等多种耐药菌对人类医疗保健系统构成挑战。MRSA 是医院获得性和社区感染的主要原因之一。
因此，在本研究中，我们评估了由辛伐他汀合成的银纳米粒子（AgNP）与辛伐他汀联合对参考和多药耐药细菌株的抗菌活性。
辛伐他汀对 MRSA 的最小抑菌浓度（MIC）范围为 0.062 至 0.25mg/mL。大小为 77.68±33.95nm，zeta 电位为-34.6±12.7mV 的 AgNP 对包括 MRSA 株在内的细菌的 MIC 为 0.212mg/mL。棋盘试验和时间杀伤曲线显示辛伐他汀-AgNP 联合对 MRSA 株的抗菌活性具有协同作用。辛伐他汀和 AgNP 的联合对产 ESBL 的细菌具有抗菌活性。扫描电子显微镜显示 AgNP 处理后形成细胞表面突起，辛伐他汀处理后形成大的无定形物质，均在 MRSA 中。
我们的结果表明，AgNP 和辛伐他汀的联合可能是控制细菌感染的未来替代方法，当与辛伐他汀联合使用时，AgNP 的剂量更小，具有相同的抗菌活性。
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