State Key Laboratory of Microbial Metabolism and School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China; Biomass Energy Research Center, Shanghai Jiao Tong University, Shanghai 200240, China.
State Key Laboratory of Microbial Metabolism and School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China; Biomass Energy Research Center, Shanghai Jiao Tong University, Shanghai 200240, China.
Bioresour Technol. 2017 Mar;227:214-220. doi: 10.1016/j.biortech.2016.12.039. Epub 2016 Dec 18.
Switching cultivation (mixotrophic-heterotrophic, 12h:12h) of Heynigia riparia SX01 was studied, the maximum biomass concentration of 3.55gL and lipid yield of 1.45gL were achieved after 8days cultivation. The extracellular polymeric substance (EPS) was developed as co-product. Addition of MgSO could enhance the production of EPS. The highest amount of 0.60gL EPS was obtained with the addition of 2gL MgSO, the self-flocculation efficiency was as high as 83% at this condition. The total lipid and lipid fractions did not show differences with extra MgSO. Based on the above results, a new biodiesel production model was proposed: culturing Heynigia riparia SX01 with extra 2gL MgSO by switching cultivation and using self flocculation to collect microalgae for biodiesel production, while EPS was collected as valuable co-products.
交替培养（混养-异养，12h:12h）对 ripariaSX01 进行了研究，经过 8 天的培养，最大生物量浓度达到 3.55gL，产油量达到 1.45gL。胞外聚合物（EPS）作为副产物被开发出来。添加硫酸镁可以提高 EPS 的产量。在添加 2gL 的硫酸镁的条件下，获得了最高的 0.60gL EPS，自絮凝效率高达 83%。在这种条件下，总脂质和脂质组分与额外的 MgSO 没有差异。基于上述结果，提出了一种新的生物柴油生产模型：通过交替培养用额外的 2gL MgSO 培养 Heynigia ripariaSX01，并利用自絮凝收集微藻用于生产生物柴油，同时收集 EPS 作为有价值的副产物。
