Chug Ravneet, Mathur Shruti, Kothari S L, Gour Vinod Singh
Amity Institute of Biotechnology, Amity University Rajasthan, NH 11C, Kant Kalwar, Jaipur, 303002, India.
Department of Botany, Mohanlal Sukhadia University Udaipur, Rajasthan, India.
Biochem Biophys Rep. 2021 Mar 18;26:100972. doi: 10.1016/j.bbrep.2021.100972. eCollection 2021 Jul.
Heavy metal contamination of water bodies has been a cause of grave concern around the globe. Analysis of various industrial effluents has revealed a perilous level of Cr (VI) and Ni (II). is an extracellular polymeric substances (EPSs) producing bacterium. EPS has a great potential in the sequestration of heavy metal ions. In the present study efforts have been made to understand the effect of time, pH, and temperature on production of EPS by (MTCC 1688). The extracted EPS has been applied for removal of Ni (II) and Cr (VI) ions from aqueous system. The results revealed that highest EPS yield (26 mg/50 mL) can be obtained after 96 h of incubation at pH 6 and 32 °C temperature in 50 mL of culture. Treatment of 10 mg/L Cr (VI) and Ni (II) with 30 mg/L EPS resulted in the removal of 26% and 9% of Cr (VI) and Ni (II), respectively. Fourier-transform infrared spectral analysis revealed the involvement of -OH, -NH, C-O, diketone, and ester functional groups of EPS in the attachment of Cr (VI) ion while involvement of amide and -C[bond, double bond]O groups in Ni (II) binding with EPS. Scaling-up the production of EPS using bioreactor may further help in developing an efficient process for treatment of water polluted with Cr and Ni.
水体的重金属污染一直是全球严重关切的问题。对各种工业废水的分析显示，铬（VI）和镍（II）的含量处于危险水平。[细菌名称]是一种能产生细胞外聚合物（EPSs）的细菌。EPS在螯合重金属离子方面具有巨大潜力。在本研究中，已努力了解时间、pH值和温度对[细菌名称]（MTCC 1688）产生EPS的影响。提取的EPS已用于从水体系中去除镍（II）和铬（VI）离子。结果表明，在50 mL培养基中，于pH 6和32°C温度下培养96小时后，可获得最高的EPS产量（26 mg/50 mL）。用30 mg/L的EPS处理10 mg/L的铬（VI）和镍（II），分别去除了26%的铬（VI）和9%的镍（II）。傅里叶变换红外光谱分析表明，EPS的-OH、-NH、C-O、二酮和酯官能团参与了铬（VI）离子的附着，而酰胺和-C=O基团参与了镍（II）与EPS的结合。使用生物反应器扩大EPS的生产可能进一步有助于开发一种高效的工艺来处理受铬和镍污染的水。
