Department of Gastrointestinal Surgery, Laboratory Research Center, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, PR China.
Department of General Surgery, DaZhou Central Hospital, Dazhou, Sichuan 635000, PR China.
Hum Pathol. 2017 Dec;70:35-42. doi: 10.1016/j.humpath.2017.02.034. Epub 2017 Jun 7.
We investigated the expression of dimethyladenosine transferase 1 homolog (DIMT1) in human gastric carcinoma (GC) tissues, pericarcinoma histologically normal tissues, and normal gastric tissues and explored its clinical significance. Immunohistochemistry staining was used to detect the expression of DIMT1, and the findings were compared with clinicopathological features of patients with GC. The result also was ascertained by Western blotting. The Kaplan-Meier method and log-rank test were used to compare the overall survival rate and time in the DIMT1 low-level and high-level expression groups. Immunohistochemical staining indicated that the expression of DIMT1 in GC tissues (65/75; 86.7%) was significantly more common (P<.001) than that in pericarcinoma histologically normal tissues (14/75; 18.7%) and normal gastric tissues (2/12; 16.7%). High expression of DIMT1 correlated closely with differentiation (P=.023), invasion (P=.042), lymph node metastasis (P=.008), distant metastasis (P=.006), and TNM stage (P=.013). Western blotting showed that DIMT1 expression correlated positively with TNM stage and implied that more advanced TNM stage was accompanied by higher expression of DIMT1 (P<.001). Kaplan-Meier survival analysis showed that high DIMT1 expression correlated significantly (P<.001) with a poor prognosis. Our data suggest that DIMT1 is a useful molecular biomarker to predict tumor progression and prognosis in patients with GC.
我们研究了二甲基腺苷转移酶 1 同源物（DIMT1）在人胃癌（GC）组织、癌旁组织和正常胃组织中的表达，并探讨了其临床意义。采用免疫组织化学染色法检测 DIMT1 的表达，并与 GC 患者的临床病理特征进行比较。采用 Western blot 进一步确定结果。采用 Kaplan-Meier 法和对数秩检验比较 DIMT1 低表达和高表达组的总生存率和时间。免疫组化染色显示，GC 组织中 DIMT1 的表达（65/75；86.7%）明显高于癌旁组织（14/75；18.7%）和正常胃组织（2/12；16.7%）（P<.001）。DIMT1 的高表达与分化（P=.023）、浸润（P=.042）、淋巴结转移（P=.008）、远处转移（P=.006）和 TNM 分期（P=.013）密切相关。Western blot 显示 DIMT1 的表达与 TNM 分期呈正相关，提示更晚期的 TNM 分期伴随着更高的 DIMT1 表达（P<.001）。Kaplan-Meier 生存分析显示，高 DIMT1 表达与预后不良显著相关（P<.001）。我们的数据表明，DIMT1 是预测 GC 患者肿瘤进展和预后的有用分子标志物。
