Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand; Center of Excellence in Emerging and Re-emerging Diarrheal Viruses, Chiang Mai University, Chiang Mai, Thailand.
Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine, Tokyo, Japan.
Infect Genet Evol. 2018 Nov;65:357-368. doi: 10.1016/j.meegid.2018.08.019. Epub 2018 Aug 23.
Two rare human rotavirus strains, RVA/Human-wt/THA/CMH-N016-10/2010/G4P[6] and RVA/Human-wt/THA/CMH-N014-11/2011/G4P[6], were detected during the surveillance of group A rotavirus (RVA) in Chiang Mai, Thailand. Complete genome sequences of both strains were analyzed in comparison with that of the representative porcine G4P[6] RVA strain (RVA/Pig-wt/THA/CMP-011-09/2009/G4P[6]) detected in the same geographical area. Human RVA strain CMH-N016-10 containing the genotype constellation of G4-P[6]-I1-R1-C1-M1-A8-N1-T1-E1-H1 was identical to that of porcine RVA strain CMP-011-09. Another human RVA strain (CMH-N014-11) was also contained the genotype constellation of ten segments identical to those of CMH-N016-10 and of porcine RVA strain CMP-011-09 except for genotype I of VP6 gene which contained I5 instead of I1. The genotype constellation of CMH-N014-11, G4-P[6]-I5-R1-C1-M1-A8-N1-T1-E1-H1 was a novel genotype constellation that has not been reported previously in both human and pig. Phylogenetic analysis of all 11 genome segments revealed that both strains of human RVA were more closely related to porcine and porcine-like human than to human RVA reference strains, particularly those reported from Thailand and other Asian countries with very high nucleotide sequence identities ranging from 91.1-100% except for NSP4 gene from 86.1-92.2%. Based on complete genome constellation and overall phylogenetic analyses suggested that these two human G4P[6] strains may have probably originated from porcine RVA strains of independent ancestor. This study provided an evidence for direct interspecies transmission of porcine RVA from pig to human.
在泰国清迈进行的 A 组轮状病毒（RVA）监测中，发现了两种罕见的人类轮状病毒株，RVA/Human-wt/THA/CMH-N016-10/2010/G4P[6]和 RVA/Human-wt/THA/CMH-N014-11/2011/G4P[6]。对这两种病毒株的全基因组序列进行了分析，并与在同一地理区域检测到的代表猪 G4P[6]RVA 株（RVA/Pig-wt/THA/CMP-011-09/2009/G4P[6]）进行了比较。人类 RVA 株 CMH-N016-10 含有 G4-P[6]-I1-R1-C1-M1-A8-N1-T1-E1-H1 的基因型组合，与猪 RVA 株 CMP-011-09 相同。另一种人类 RVA 株（CMH-N014-11）也含有与 CMH-N016-10 和猪 RVA 株 CMP-011-09 相同的十个片段的基因型组合，但 VP6 基因的基因型 I 含有 I5 而不是 I1。CMH-N014-11 的基因型组合，G4-P[6]-I5-R1-C1-M1-A8-N1-T1-E1-H1 是一种新的基因型组合，以前在人和猪中均未报道过。对所有 11 个基因组片段的系统进化分析表明，这两种人类 RVA 株与猪和类猪的人类 RVA 株更为密切相关，而与来自泰国和其他亚洲国家的人类 RVA 参考株关系较远，除 NSP4 基因外，核苷酸序列同一性非常高，范围为 91.1-100%，而 NSP4 基因的核苷酸序列同一性为 86.1-92.2%。基于完整的基因组组合和整体系统发育分析，提示这两种人类 G4P[6]株可能来源于独立祖先的猪 RVA 株。本研究为猪 RVA 从猪直接传播给人类提供了证据。
