Punt C J, Rijksen G, Vlug A M, Dekker A W, Staal G E
Department of Medical Oncology, Dr Daniel den Hoed Cancer Center, Rotterdam, The Netherlands.
Br J Haematol. 1989 Sep;73(1):51-6. doi: 10.1111/j.1365-2141.1989.tb00219.x.
Tyrosine protein kinase (TPK) activity was measured in subcellular fractions of normal granulocytes, lymphocytes and monocytes, and acute and chronic myeloid and lymphoid leukaemic cells. Of several tested tyrosine-containing substrates, poly (glutamic acid: tyrosine = 4:1) (S1) proved to be the best synthetic substrate. High cytosolic TPK activity was found in every cell type. Different TPKs may exist in various cell fractions, as was indicated by the difference in Km values for S1 in cell fractions of normal granulocytes and lymphocytes. No significant difference was found in total TPK activity between normal and leukaemic cells, indicating that total TPK activity is not related to the leukaemic process itself. A highly significant difference was found in membrane fractions in normal granulocytes and M1-M2 AML cells versus normal monocytes and M4-M5 AML cells, suggesting an association between TPK activity and monocytic differentiation in these cell fractions.
在正常粒细胞、淋巴细胞和单核细胞以及急性和慢性髓系及淋巴系白血病细胞的亚细胞组分中测量了酪氨酸蛋白激酶（TPK）活性。在几种经过测试的含酪氨酸底物中，聚（谷氨酸：酪氨酸 = 4:1）（S1）被证明是最佳合成底物。在每种细胞类型中均发现了较高的胞质TPK活性。正常粒细胞和淋巴细胞的细胞组分中S1的Km值存在差异，这表明不同的TPK可能存在于各种细胞组分中。正常细胞和白血病细胞之间的总TPK活性未发现显著差异，这表明总TPK活性与白血病过程本身无关。在正常粒细胞与M1 - M2急性髓系白血病（AML）细胞的膜组分与正常单核细胞和M4 - M5 AML细胞之间发现了高度显著差异，这表明在这些细胞组分中TPK活性与单核细胞分化之间存在关联。
