Kuratsune H, Owada K, Machii T, Nishimori Y, Ueda E, Tokumine Y, Tagawa S, Taniguchi N, Fujio H, Kitani T
Biochem Biophys Res Commun. 1985 Dec 17;133(2):598-607. doi: 10.1016/0006-291x(85)90947-7.
In vitro protein phosphorylation in various types of human fresh lymphoid leukemic cells (C-ALL, B-CLL, HCL and PCL: B-cell lineage and T-ALL, ATL and T-CLL: T-cell lineage) were studied. In cases of B-CLL and HCL, tyrosine protein kinase (TPK) activity was at least 5-fold higher than that in other cases of B- and T-cell lineages. B-cell leukemic cells at various differentiation stages had different endogenous substrates in tyrosine phosphorylation as well as distinct TPK activity. The P-tyr-containing proteins of 68K, 59K and 56K were detected commonly in all the cases of B-cell lineage. The phosphorylated protein of 32K was present only in cases of PCL. On the other hand, in T-ALL and ATL, the major substrate in tyrosine phosphorylation was 58K. These results suggest that the characterization of in vitro tyrosine phosphorylation provides a new means not only to distinguish T- and B-lymphoid leukemia, but also to differentiate stages of lymphoid development.
研究了各种类型人类新鲜淋巴细胞白血病细胞（普通型急性淋巴细胞白血病、B细胞慢性淋巴细胞白血病、毛细胞白血病和幼淋巴细胞白血病：B细胞系；T细胞急性淋巴细胞白血病、成人T细胞白血病和T细胞慢性淋巴细胞白血病：T细胞系）中的体外蛋白质磷酸化情况。在B细胞慢性淋巴细胞白血病和毛细胞白血病病例中，酪氨酸蛋白激酶（TPK）活性比其他B细胞系和T细胞系病例至少高5倍。处于不同分化阶段的B细胞白血病细胞在酪氨酸磷酸化方面具有不同的内源性底物以及不同的TPK活性。在所有B细胞系病例中均普遍检测到68K、59K和56K含酪氨酸磷酸化蛋白。32K磷酸化蛋白仅存在于幼淋巴细胞白血病病例中。另一方面，在T细胞急性淋巴细胞白血病和成人T细胞白血病中，酪氨酸磷酸化的主要底物是58K。这些结果表明，体外酪氨酸磷酸化的特征不仅为区分T淋巴细胞白血病和B淋巴细胞白血病提供了一种新方法，也为区分淋巴细胞发育阶段提供了新方法。
