Akyol Alpaslan, Şimşek Memet, İlhan Raşit, Can Behzat, Baspinar Melike, Akyol Hadice, Gül H Fatih, Gürsu Ferit, Kavak Burçin, Akın Mustafa
Department of Obstetrics and Gynecology, Firat University School of Medicine, Elazig, Turkey.
Department of Obstetrics and Gynecology, Firat University School of Medicine, Elazig, Turkey.
Taiwan J Obstet Gynecol. 2016 Dec;55(6):835-839. doi: 10.1016/j.tjog.2015.06.018.
The aim of this study was to investigate the effects of 1,25-dihydroxyvitamin-D3 (vitamin D) and omega-3 polyunsaturated fatty acids (omega-3 PUFA) on experimentally induced endometriosis in a rat model.
A prospective, single-blind, randomized, controlled experimental study was performed on 30 Wistar female rats. Endometriosis was surgically induced by implanting endometrial tissue on the abdominal peritoneum. Four weeks later, a second laparotomy was performed to assess pre-treatment implant volumes and cytokine levels. The rats were randomized into three groups: vitamin D group (42 μg/kg/day), omega-3 PUFA group (450 mg/kg/day), and control group (saline 0.1 mL/rat/day). These treatments were administered for 4 weeks. At the end of treatment, a third laparotomy was performed for the assessment of cytokine levels, implant volumes (post-treatment) and implants were totally excised for histopathologic examination. Pre- and post-treatment volumes, cytokine levels within the groups, as well as stromal and glandular tissues between the groups were compared.
The mean post-treatment volume was statistically significantly reduced in the omega-3 PUFA group (p=0.02) and the level of the interleukin-6 (IL-6), tumor necrosis factor alpha (TNF-α), vascular endothelial growth factor (VEGF) in the peritoneal fluid were significantly decreased at the end of treatment in the omega-3 PUFA group (p=0.02, p=0.03, and p=0.03, respectively). In the vitamin D group, only IL-6 levels were significantly decreased. In the histopathologic examination, the glandular tissue and stromal tissue scores of the implants were significant lower in the omega-3 PUFA group (p=0.03 and p=0.02).
Omega-3 PUFA caused significant regression of endometriotic implants. Vitamin D has not been as effective as omega-3 PUFA on endometriosis.
本研究旨在探讨1,25 - 二羟基维生素D3（维生素D）和ω-3多不饱和脂肪酸（ω-3 PUFA）对大鼠实验性诱导子宫内膜异位症的影响。
对30只Wistar雌性大鼠进行了一项前瞻性、单盲、随机、对照实验研究。通过将子宫内膜组织植入腹部腹膜来手术诱导子宫内膜异位症。四周后，进行第二次剖腹手术以评估治疗前植入物体积和细胞因子水平。大鼠被随机分为三组：维生素D组（42μg/kg/天）、ω-3 PUFA组（450mg/kg/天）和对照组（0.1mL/大鼠/天生理盐水）。这些治疗持续4周。治疗结束时，进行第三次剖腹手术以评估细胞因子水平、植入物体积（治疗后），并将植入物完全切除进行组织病理学检查。比较了组内治疗前后的体积、细胞因子水平，以及组间的基质和腺体组织。
ω-3 PUFA组治疗后的平均体积在统计学上显著减小（p = 0.02），且治疗结束时ω-3 PUFA组腹腔液中白细胞介素-6（IL-6）、肿瘤坏死因子α（TNF-α）、血管内皮生长因子（VEGF）水平显著降低（分别为p = 0.02、p = 0.03和p = 0.03）。在维生素D组，仅IL-6水平显著降低。在组织病理学检查中，ω-3 PUFA组植入物的腺体组织和基质组织评分显著更低（p = 0.03和p = 0.02）。
ω-3 PUFA可使子宫内膜异位植入物显著消退。维生素D对子宫内膜异位症的效果不如ω-3 PUFA。
