Ito Kazuyo, Yoshida Kenji, Maruyama Hitoshi, Mamou Jonathan, Yamaguchi Tadashi
Graduate School of Engineering, Chiba University, Inage, Chiba, Chiba, Japan.
Center for Frontier Medical Engineering, Chiba University, Inage, Chiba, Chiba, Japan.
Ultrasound Med Biol. 2017 Mar;43(3):700-711. doi: 10.1016/j.ultrasmedbio.2016.11.011. Epub 2016 Dec 28.
Acoustic properties of free fatty acids present in the liver were studied as a possible basis for non-invasive ultrasonic diagnosis of non-alcoholic steatohepatitis. Acoustic impedance was measured for the following types of tissue samples: Four pathologic types of mouse liver, five kinds of FFAs in solvent and five kinds of FFAs in cultured Huh-7 cells. A transducer with an 80-MHz center frequency was incorporated into a scanning acoustic microscopy system. Acoustic impedance was calculated from the amplitude of the signal reflected from the specimen surface. The Kruskal-Wallis test revealed statistically significant differences (p < 0.01) in acoustic impedance not only among pathologic types, but also among the FFAs in solvent and in cultured Huh-7 cells. These results suggest that each of the FFAs, especially palmitate, oleate and palmitoleate acid, can be distinguished from each other, regardless of whether they were in solution or absorbed by cells.
研究了肝脏中游离脂肪酸的声学特性，作为非酒精性脂肪性肝炎非侵入性超声诊断的可能依据。对以下类型的组织样本测量了声阻抗：四种病理类型的小鼠肝脏、溶剂中的五种游离脂肪酸以及培养的Huh-7细胞中的五种游离脂肪酸。将中心频率为80 MHz的换能器集成到扫描声学显微镜系统中。根据从样本表面反射的信号幅度计算声阻抗。Kruskal-Wallis检验显示，不仅在病理类型之间，而且在溶剂中和培养的Huh-7细胞中的游离脂肪酸之间，声阻抗存在统计学上的显著差异（p < 0.01）。这些结果表明，每种游离脂肪酸，尤其是棕榈酸、油酸和棕榈油酸，无论它们是处于溶液中还是被细胞吸收，都可以相互区分。
