Matarashvili Iza, Ghughunishvili Darejan, Chankvetadze Lali, Takaishvili Nino, Khatiashvili Tamar, Tsintsadze Maia, Farkas Tivadar, Chankvetadze Bezhan
Institute of Physical and Analytical Chemistry, School of Exact and Natural Sciences, Tbilisi State University, Chavchavadze Ave 3, 0179 Tbilisi, Georgia.
Faculty of Chemistry, Georgian Technical University, Kostava Str. 77, 0102 Tbilisi, Georgia.
J Chromatogr A. 2017 Feb 3;1483:86-92. doi: 10.1016/j.chroma.2016.12.064. Epub 2016 Dec 22.
When polysaccharide-based chiral columns are used in combination with aqueous-organic mobile phases for the separation of enantiomers in high-performance liquid chromatography the separation mode is commonly called "reversed-phase" in analogy to achiral separations. In several earlier and recent studies on neutral and basic chiral analytes it was shown by our and other groups that due to multiple type of interactions involved in selector-selectand binding and enantioselective recognition with polysaccharide derivatives, the above mentioned separation system may not always behave like a reversed-phase system. In the present study additional examples of non-reversed-phase behavior are described for the first time for weak acidic chiral analytes. In addition, the reversal of enantiomer elution order was observed again for the first time for several analytes based on water-content in the mobile phase.
当基于多糖的手性柱与水-有机流动相结合用于高效液相色谱中对映体的分离时，这种分离模式通常类似于非手性分离被称为“反相”。在我们小组和其他小组关于中性和碱性手性分析物的一些早期及近期研究中发现，由于多糖衍生物在选择剂-被选择物结合以及对映体选择性识别过程中涉及多种相互作用类型，上述分离系统可能并不总是表现得像反相系统。在本研究中，首次描述了弱酸性手性分析物非反相行为的更多实例。此外，首次再次观察到几种分析物的对映体洗脱顺序会基于流动相中的含水量发生反转。
