Departamento de Química Analítica, Universitat de València, Burjassot, Valencia, Spain.
Departamento de Química Analítica, Universitat de València, Burjassot, Valencia, Spain.
J Chromatogr A. 2020 Aug 16;1625:461334. doi: 10.1016/j.chroma.2020.461334. Epub 2020 Jun 10.
A comprehensive study on the chiral separation of bupivacaine, mepivacaine, prilocaine and propanocaine with eight commercial polysaccharide-based chiral stationary phases (CSPs) in reversed phase conditions compatible with MS detection is performed. Methanol and acetonitrile are used as organic modifiers. Retention and resolution values obtained for each compound in the different CSPs and mobile phases are compared. The polysaccharide-based CSPs tested present different enantioselectivity towards the analytes. From the results, the experimental conditions for determining the enantiomers of bupivacaine, mepivacaine, prilocaine and propanocaine in saline aqueous samples using MS detection are used, for the first time, to perform an enantioselective biodegradability study.
采用 8 种商品化多糖手性固定相（CSP）在反相条件下对布比卡因、甲哌卡因、丙胺卡因和普鲁卡因进行了手性拆分的综合研究，这些条件与 MS 检测兼容。使用甲醇和乙腈作为有机改性剂。比较了在不同 CSP 和流动相下每种化合物的保留和分离值。所测试的基于多糖的 CSP 对分析物表现出不同的对映选择性。根据结果，首次使用这些实验条件在盐水中样品中使用 MS 检测来测定布比卡因、甲哌卡因、丙胺卡因和普鲁卡因对映体，并进行了对映体选择性生物降解性研究。
