Diéguez Ana L, Balboa Sabela, Magnesen Thorolf, Romalde Jesús L
Departamento de Microbiología y Parasitología, CIBUS-Facultad de Biología, Universidade de Santiago, 15782 Santiago de Compostela, Spain.
Department of Biology, Faculty of Mathematics and Natural Sciences, University of Bergen, Bergen, Norway.
Syst Appl Microbiol. 2017 Mar;40(2):80-85. doi: 10.1016/j.syapm.2016.12.002. Epub 2016 Dec 21.
Nine isolates obtained from a great scallop hatchery in Norway were characterized using a polyphasic approach. Strains were Gram-negative, aerobic and motile rods with oxidative metabolism. Phylogenetic analysis based on the sequences of 16S rRNA and rpoB genes showed that these strains formed two different groups associated with members of the genus Neptuniibacter. DNA-DNA hybridization (DDH) and Average Nucleotide Identity (ANI) demonstrated that the isolates constituted two novel species of this genus, which can be phenotypically differentiated from their closest relatives. The names Neptuniibacter marinus sp. nov. and Neptuniibacter pectenicola sp. nov are proposed, with ATR 1.1 (=CECT 8938=DSM 100783) and LFT 1.8 (=CECT 8936=DSM 100781) as respective type strains.
采用多相分类法对从挪威一家大扇贝孵化场获得的9株菌株进行了鉴定。这些菌株为革兰氏阴性、需氧且具运动性的杆菌，具有氧化代谢。基于16S rRNA和rpoB基因序列的系统发育分析表明，这些菌株形成了与海杆菌属成员相关的两个不同组。DNA-DNA杂交（DDH）和平均核苷酸同一性（ANI）表明，这些分离株构成了该属的两个新物种，它们在表型上可与其最亲近的亲缘种区分开来。提出了新物种海海杆菌（Neptuniibacter marinus sp. nov.）和栉孔扇贝海杆菌（Neptuniibacter pectenicola sp. nov），分别以ATR 1.1（=CECT 8938=DSM 100783）和LFT 1.8（=CECT 8936=DSM 100781）作为模式菌株。
