Neptuniibacter 属的基因组分类学及从海参幼体中分离得到的新种 Neptuniibacter victor 的提出。
Genome taxonomy of the genus Neptuniibacter and proposal of Neptuniibacter victor sp. nov. isolated from sea cucumber larvae.
机构信息
Laboratory of Microbiology, Faculty of Fisheries Sciences, Hokkaido University, Hakodate, Japan.
Aquaculture Department, Faculty of Fisheries and Marine Sciences, Universitas Diponegoro, Semarang, Indonesia.
出版信息
PLoS One. 2023 Aug 15;18(8):e0290060. doi: 10.1371/journal.pone.0290060. eCollection 2023.
A Gram-staining-negative, oxidase-positive, strictly aerobic rod-shaped bacterium, designated strain PT1T, was isolated from the laboratory-reared larvae of the sea cucumber Apostichopus japonicus. A phylogenetic analysis based on the 16S rRNA gene nucleotide sequences revealed that PT1T was closely related to Neptuniibacter marinus ATR 1.1T (= CECT 8938T = DSM 100783T) and Neptuniibacter caesariensis MED92T (= CECT 7075T = CCUG 52065T) showing 98.2% and 98.1% sequence similarity, respectively. However, the average nucleotide identity (ANI) and in silico DNA-DNA hybridization (DDH) values among these three strains were 72.0%-74.8% and 18.3%-19.5% among related Neptuniibacter species, which were below 95% and 70%, respectively, confirming the novel status of PT1T. The average amino acid identity (AAI) values of PT1T showing 74-77% among those strains indicated PT1T is a new species in the genus Neptuniibacter. Based on the genome-based taxonomic approach, Neptuniibacter victor sp. nov. is proposed for PT1T. The type strain is PT1T (JCM 35563T = LMG 32868T).
一株革兰氏阴性、氧化酶阳性、严格需氧的杆状细菌，被命名为 PT1T 菌株，从人工养殖的海参（Apostichopus japonicus）幼虫中分离得到。基于 16S rRNA 基因核苷酸序列的系统发育分析表明，PT1T 与 Neptuniibacter marinus ATR 1.1T（= CECT 8938T = DSM 100783T）和 Neptuniibacter caesariensis MED92T（= CECT 7075T = CCUG 52065T）密切相关，序列相似性分别为 98.2%和 98.1%。然而，这三个菌株之间的平均核苷酸同一性（ANI）和 DNA-DNA 杂交（DDH）值在 72.0%-74.8%和 18.3%-19.5%之间，与相关 Neptuniibacter 种之间的 95%和 70%分别低于确认 PT1T 的新状态。PT1T 的平均氨基酸同一性（AAI）值在这些菌株中为 74-77%，表明 PT1T 是 Neptuniibacter 属的一个新种。基于基于基因组的分类方法，提议将 PT1T 命名为 Neptuniibacter victor sp. nov. 其模式菌株为 PT1T（JCM 35563T = LMG 32868T）。
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