Lima Luciane Mota, Cardoso Luciana Santos, Santos Silvane Braga, Oliveira Ricardo Riccio, Oliveira Sérgio Costa, Góes Alfredo Miranda, Loukas Alex, Araujo Maria Ilma
Serviço de Imunologia, Hospital Universitário Professor Edgard Santos, Brazil.
Serviço de Imunologia, Hospital Universitário Professor Edgard Santos, Brazil; Departamento de Análises Clínicas e Toxicológicas, Faculdade de Farmácia, Universidade Federal da Bahia, Salvador, Bahia, Brazil; Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais - INCT-DT (CNPq/MCT), Brazil.
Acta Trop. 2017 Mar;167:157-162. doi: 10.1016/j.actatropica.2016.12.030. Epub 2016 Dec 28.
HTLV-1 is the causal agent of Adult T cell Leukemia/lymphoma (ATLL) and HTLV-1-associated Myelopathy/Tropical Spastic Paraparesis (HAM/TSP). The immune response to HTLV-1-infection is polarized to the Th1-type, and the presence of CXCL9/CXCL10 chemokines may lead to an increase in the recruitment of pro-inflammatory molecules in spinal cord tissue, contributing to the damage observed in the development of HAM/TSP. It has been observed that in chronic helminth-infections, such as schistosomiasis, there is a deviation toward the Th2/regulatory immune response.
To evaluate the ability of Schistosoma spp. proteins to decrease the in vitro CXCL9 and CXCL10 production by PBMC of HTLV-1-infected individuals.
The Schistosoma proteins rSm29, rSh-TSP-2 and PIII were added to PBMC cultures of HTLV-1-infected individuals and the levels of chemokines in the supernatants were measured using a sandwich ELISA method.
The addition of rSm29 to the cultures resulted in decreased production of CXCL9 in all the analyzed individuals and HAM/TSP group (18167±9727pg/mL, p=0.044; 20237±6023pg/mL, p=0.028, respectively) compared to the levels in unstimulated cultures (19745±9729pg/mL; 25078±2392pg/mL, respectively). The addition of rSh-TSP-2 decreased the production of CXCL9 in all studied individuals and carriers group (16136±9233pg/mL, p=0.031; 13977±8857pg/mL, p=0.026) vs unstimulated cultures (19745±9729pg/mL; 18121±10508pg/mL, respectively). Addition of PIII did not alter the results. There was no significant change in the levels of CXCL10 by the addition of the studied proteins.
The Schistosoma proteins used in this study were able to down modulate the production of CXCL9, a chemokine associated with the inflammatory process in HTLV-1-infection.
人类嗜T淋巴细胞病毒1型（HTLV-1）是成人T细胞白血病/淋巴瘤（ATLL）和HTLV-1相关脊髓病/热带痉挛性截瘫（HAM/TSP）的致病因子。对HTLV-1感染的免疫反应偏向Th1型，CXCL9/CXCL10趋化因子的存在可能导致脊髓组织中促炎分子募集增加，促使在HAM/TSP发展过程中观察到的损伤。据观察，在慢性蠕虫感染（如血吸虫病）中，免疫反应会偏向Th2/调节性免疫反应。
评估血吸虫属蛋白降低HTLV-1感染个体外周血单个核细胞（PBMC）体外CXCL9和CXCL10产生的能力。
将血吸虫蛋白rSm29、rSh-TSP-2和PIII添加到HTLV-1感染个体的PBMC培养物中，采用夹心酶联免疫吸附测定法测量上清液中趋化因子的水平。
与未刺激培养物中的水平（分别为19745±9729pg/mL；25078±2392pg/mL）相比，向培养物中添加rSm29导致所有分析个体和HAM/TSP组中CXCL9的产生减少（分别为18167±9727pg/mL，p=0.044；20237±6023pg/mL，p=0.028）。添加rSh-TSP-2使所有研究个体和携带者组中CXCL9的产生减少（分别为16136±9233pg/mL，p=0.031；13977±8857pg/mL，p=0.026），而未刺激培养物中的水平分别为19745±9729pg/mL；18121±10508pg/mL。添加PIII未改变结果。添加所研究的蛋白后，CXCL10水平无显著变化。
本研究中使用的血吸虫蛋白能够下调CXCL9的产生，CXCL9是一种与HTLV-1感染炎症过程相关的趋化因子。
