Palomar Gemma, Ahmad Freed, Vasemägi Anti, Matsuba Chikako, Nicieza Alfredo G, Cano José Manuel
Research Unit of Biodiversity (University of Oviedo-Consejo Superior de Investigaciones Científicas-Principado de Asturias), University of Oviedo, 33600 Mieres, Asturias, Spain
Department of Biology of Organisms and Systems, University of Oviedo, 33006, Asturias, Spain.
G3 (Bethesda). 2017 Feb 9;7(2):637-645. doi: 10.1534/g3.116.036459.
By combining 7077 SNPs and 61 microsatellites, we present the first linkage map for some of the early diverged lineages of the common frog, , and the densest linkage map to date for this species. We found high homology with the published linkage maps of the Eastern and Western lineages but with differences in the order of some markers. Homology was also strong with the genome of the Tibetan frog and we found high synteny with the clawed frog We confirmed marked heterochiasmy between sexes and detected nonrecombining regions in several groups of the male linkage map. Contrary to the expectations set by the male heterogamety of the common frog, we did not find male heterozygosity excess in the chromosome previously shown to be linked to sex determination. Finally, we found blocks of loci showing strong transmission ratio distortion. These distorted genomic regions might be related to genetic incompatibilities between the parental populations, and are promising candidates for further investigation into the genetic basis of speciation and adaptation in the common frog.
通过结合7077个单核苷酸多态性（SNP）和61个微卫星，我们绘制出了普通青蛙一些早期分化谱系的首张连锁图谱，也是该物种迄今为止最密集的连锁图谱。我们发现它与已发表的东方和西方谱系连锁图谱具有高度同源性，但某些标记的顺序存在差异。与西藏蟾蜍的基因组也有很强的同源性，并且我们发现与爪蟾有高度的同线性。我们证实了两性之间明显的交叉差异，并在雄性连锁图谱的几组中检测到非重组区域。与普通青蛙雄性异配性所设定的预期相反，我们在先前显示与性别决定相关的染色体上未发现雄性杂合性过剩。最后，我们发现了一些显示出强烈传递率失真的基因座区域。这些扭曲的基因组区域可能与亲本种群之间的遗传不相容性有关，是进一步研究普通青蛙物种形成和适应的遗传基础的有前景的候选对象。
