The level of beta defensin-2 in saliva and its expression in parotid gland epithelial cells after probiotic () induction to inhibit in caries.

OBJECTIVE

The aim of this study was to prove that administrating probiotics can increase the level of BD-2 saliva and BD-2 expression in the epithelial parotid glands of Wistar rats.

MATERIALS AND METHODS

Experimental design in this study was randomized control group post test only. Twenty-four white male Wistar strain rats were divided into four groups. The negative control group included rats not induced by whereas the positive control group included rats induced by . The two treatment groups are as follows: treatment 1 (T1), the group that is induced for 14 days by and 7 days by and treatment 2 (T2), the group which is induced simultaneously by and for 14 days. culture at a concentration of 108 colony-forming unit/ml and culture at a concentration of 1010 are induced in the oral cavity of the Wistar rats. The Elisa technique is used to examine the salivary level of BD-2, whereas the immunohistochemical technique is used to examine the BD-2 expression in the epithelial salivary glands.

RESULTS

The study shows the increasing levels of BD-2 and BD-2 expression in the epithelial parotid glands after the administration of probiotics. Besides, there is a relationship between the increasing expression of BD-2 in the epithelial parotid with the decreasing amount of .

CONCLUSION

Giving probiotic scan increases the level of saliva of BD-2 and the expression of BD-2 in the parotid glands.