Lai Tongfei, Wang Ying, Fan Yaya, Zhou Yingying, Bao Ying, Zhou Ting
Key Laboratory for Quality and Safety of Agricultural Products of Hangzhou City, College of Life and Environmental Science, Hangzhou Normal University, Hangzhou 310036, China; Research Centre for Plant RNA Signaling, College of Life and Environmental Science, Hangzhou Normal University, Hangzhou 310036, China.
Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China; University of Chinese Academy of Sciences, Beijing 100093, China.
Int J Food Microbiol. 2017 Mar 6;244:1-10. doi: 10.1016/j.ijfoodmicro.2016.12.017. Epub 2016 Dec 28.
In this study, the effects of exogenous potassium phosphite (Phi) on growth and patulin production of postharvest pathogen Penicillium expansum were assessed. The results indicated that P. expansum under 5mmol/L Phi stress presented obvious development retardation, yield reduction of patulin and lower infectivity to apple fruit. Meanwhile, expression analysis of 15 genes related to patulin biosynthesis suggested that Phi mainly affected the early steps of patulin synthetic route at transcriptional level. Furthermore, a global view of proteome and transcriptome alteration of P. expansum spores during 6h of Phi stress was evaluated by iTRAQ (isobaric tags for relative and absolute quantitation) and RNA-seq (RNA sequencing) approaches. A total of 582 differentially expressed proteins (DEPs) and 177 differentially expressed genes (DEGs) were acquired, most of which participated in carbohydrate metabolism, amino acid metabolism, lipid metabolism, genetic information processing and biosynthesis of secondary metabolites. Finally, 39 overlapped candidates were screened out through correlational analysis between iTRAQ and RNA-seq datasets. These findings will afford more precise and directional clues to explore the inhibitory mechanism of Phi on growth and patulin biosynthesis of P. expansum, and be beneficial to develop effective controlling approaches based on Phi.
在本研究中，评估了外源亚磷酸钾（Phi）对采后病原菌扩展青霉生长及棒曲霉素产生的影响。结果表明，在5mmol/L Phi胁迫下，扩展青霉呈现出明显的生长发育迟缓、棒曲霉素产量降低以及对苹果果实的侵染性减弱。同时，对15个与棒曲霉素生物合成相关基因的表达分析表明，Phi主要在转录水平上影响棒曲霉素合成途径的早期步骤。此外，通过iTRAQ（相对和绝对定量等压标签）和RNA-seq（RNA测序）方法评估了Phi胁迫6小时期间扩展青霉孢子蛋白质组和转录组变化的全局情况。共获得582个差异表达蛋白（DEP）和177个差异表达基因（DEG），其中大多数参与碳水化合物代谢、氨基酸代谢、脂质代谢、遗传信息处理和次生代谢物生物合成。最后，通过iTRAQ和RNA-seq数据集之间的相关性分析筛选出39个重叠候选物。这些发现将为探索Phi对扩展青霉生长和棒曲霉素生物合成的抑制机制提供更精确和定向的线索，并有助于开发基于Phi的有效控制方法。
