Navale Vishwambar, Vamkudoth Koteswara Rao, Ajmera Shanthipriya, Dhuri Vaibhavi
Biochemical Sciences Division, CSIR-National Chemical Laboratory, Pune, 411008, India.
Academy of Scientific and Innovative Research (AcSIR), Anusandhan Bhawan, New Delhi, India.
Toxicol Rep. 2021 May 2;8:1008-1030. doi: 10.1016/j.toxrep.2021.04.013. eCollection 2021.
species are the paramount ubiquitous fungi that contaminate various food substrates and produce biochemicals known as mycotoxins. Aflatoxins (AFTs), ochratoxin A (OTA), patulin (PAT), citrinin (CIT), aflatrem (AT), secalonic acids (SA), cyclopiazonic acid (CPA), terrein (TR), sterigmatocystin (ST) and gliotoxin (GT), and other toxins produced by species of plays a major role in food and human health. Mycotoxins exhibited wide range of toxicity to the humans and animal models even at nanomolar (nM) concentration. Consumption of detrimental mycotoxins adulterated foodstuffs affects human and animal health even trace amounts. Bioaerosols consisting of spores and hyphal fragments are active elicitors of bronchial irritation and allergy, and challenging to the public health. is the furthermost predominant environmental contaminant unswervingly defile lives with a 40-90 % mortality risk in patients with conceded immunity. Genomics, proteomics, transcriptomics, and metabolomics approaches useful for mycotoxins' detection which are expensive. Antibody based detection of toxins chemotypes may result in cross-reactivity and uncertainty. Aptamers (APT) are single stranded DNA (ssDNA/RNA), are specifically binds to the target molecules can be generated by systematic evolution of ligands through exponential enrichment (SELEX). APT are fast, sensitive, simple, in-expensive, and field-deployable rapid point of care (POC) detection of toxins, and a better alternative to antibodies.
曲霉属真菌是极为常见的真菌,会污染各种食物基质并产生被称为霉菌毒素的生化物质。黄曲霉毒素(AFTs)、赭曲霉毒素A(OTA)、展青霉素(PAT)、桔霉素(CIT)、黄曲霉震颤素(AT)、红曲霉素(SA)、环匹阿尼酸(CPA)、土曲霉毒素(TR)、柄曲霉素(ST)和 gliotoxin(GT)以及曲霉属物种产生的其他毒素在食品和人类健康中起着重要作用。霉菌毒素即使在纳摩尔(nM)浓度下也对人类和动物模型表现出广泛的毒性。食用掺有有害霉菌毒素的食品即使是微量也会影响人类和动物健康。由孢子和菌丝片段组成的生物气溶胶是支气管刺激和过敏的活跃诱发因素,对公共卫生构成挑战。曲霉属真菌是最主要的环境污染物,会持续污染生活环境,使免疫功能受损的患者有40%至90%的死亡风险。用于霉菌毒素检测的基因组学、蛋白质组学、转录组学和代谢组学方法成本高昂。基于抗体的毒素化学类型检测可能会导致交叉反应和不确定性。适体(APT)是单链DNA(ssDNA/RNA),通过指数富集配体的系统进化(SELEX)可以产生与靶分子特异性结合的适体。适体能够快速、灵敏、简单、廉价且可现场部署,用于毒素的即时快速检测(POC),是比抗体更好的选择。