Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei 106, Taiwan.
Graduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, Taipei 106, Taiwan.
Spectrochim Acta A Mol Biomol Spectrosc. 2017 Mar 15;175:239-245. doi: 10.1016/j.saa.2016.12.024. Epub 2016 Dec 18.
Surface-enhanced Raman scattering (SERS) and fluorescence microscopy are a widely used biological and chemical characterization techniques. However, the peak overlapping in multiplexed experiments and rapid photobleaching of fluorescent organic dyes is still the limitations. When compared to Ag nanocubes (NCs), higher SERS sensitivities can be obtained with thin shelled silica Ag@SiO NCs, in contrast metal-enhanced photoluminescence (MEPL) is only found with NCs that have thicker silica shells. A 'dual functionality' represented by the simultaneous strengthening of SERS and MEPL signals can be achieved by mixing Ag@SiO NCs, with a silica shell thickness of ~1.5nm and ~4.4nm. This approach allows both the Ag@SiO NCs SERS and MEPL sensitivities to be maintained at ~90% after 12weeks of storage. Based on the distinguished detection of creatinine and flavin adenine dinucleotide in the mixture, the integration of SERS and MEPL together on a stable single plasmonic nanoparticle platform offers an opportunity to enhance both biomarker detection sensitivity and specificity.
表面增强拉曼散射（SERS）和荧光显微镜是广泛使用的生物和化学特征分析技术。然而，在多路复用实验中存在的峰重叠以及荧光有机染料的快速光漂白仍然是其局限性。与银纳米立方体（NCs）相比，具有薄壳层的硅壳 Ag@SiO2 NCs 可以获得更高的 SERS 灵敏度，而只有具有较厚硅壳的 NCs 才会出现金属增强光致发光（MEPL）。通过混合具有1.5nm 和4.4nm 硅壳厚度的 Ag@SiO2 NCs，可以实现同时增强 SERS 和 MEPL 信号的“双重功能”。这种方法可以在 12 周的储存后保持 Ag@SiO2 NCs 的 SERS 和 MEPL 灵敏度约为 90%。基于混合物中肌酸和黄素腺嘌呤二核苷酸的显著检测，将 SERS 和 MEPL 集成到稳定的单个等离子体纳米颗粒平台上，为提高生物标志物检测的灵敏度和特异性提供了机会。
