Prasertbun Rapeepun, Mori Hirotake, Pintong Ai-Rada, Sanyanusin Suparut, Popruk Supaluk, Komalamisra Chalit, Changbunjong Tanasak, Buddhirongawatr Ruangrat, Sukthana Yaowalark, Mahittikorn Aongart
Department of Protozoology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Department of Helminthology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Vet Parasitol. 2017 Jan 15;233:73-79. doi: 10.1016/j.vetpar.2016.12.002. Epub 2016 Dec 5.
Enterocytozoon bieneusi is an opportunistic intestinal pathogen infecting humans and a variety of animals. Its mode of transmission and zoonotic potential are not completely understood. E. bieneusi has been frequently identified in pigs. The objective of our study was to investigate E. bieneusi in pigs and humans in Western and Central Thailand to determine its presence, genetic diversity, and zoonotic potential. A total of 277 human and 210 pig faecal samples were collected and analysed. E. bieneusi was found in 5.4% and 28.1% of human and pig samples, respectively, by nested PCR. Genotyping based on the internal transcribed spacer regions of the small subunit ribosomal RNA demonstrated three known genotypes (D, H, PigEb10) and eight novel genotypes (TMH1-8) in humans, and five known genotypes (D, EbpA, EbpC, H, O) and 11 novel genotypes (TMP1-11) in pigs. All known genotypes identified in humans and pigs had zoonotic potential. Further studies are needed to evaluate zoonotic risk of novel genotypes, as pigs may play an important role in the transmission of E. bieneusi.
微小隐孢子虫是一种感染人类和多种动物的机会性肠道病原体。其传播方式和人畜共患病潜力尚未完全明确。微小隐孢子虫在猪中经常被检测到。我们研究的目的是调查泰国西部和中部猪和人类中的微小隐孢子虫，以确定其存在情况、遗传多样性和人畜共患病潜力。共收集并分析了277份人类粪便样本和210份猪粪便样本。通过巢式PCR检测，在5.4%的人类样本和28.1%的猪样本中发现了微小隐孢子虫。基于小亚基核糖体RNA内部转录间隔区的基因分型显示，人类中有三种已知基因型（D、H、PigEb10）和八种新基因型（TMH1 - 8），猪中有五种已知基因型（D、EbpA、EbpC、H、O）和十一种新基因型（TMP1 - 11）。在人类和猪中鉴定出的所有已知基因型都具有人畜共患病潜力。由于猪可能在微小隐孢子虫的传播中起重要作用，因此需要进一步研究来评估新基因型的人畜共患病风险。
