一种用于显微镜和受激发射损耗纳米显微镜的远红光发射荧光标记蛋白，mGarnet2。

A far-red emitting fluorescent marker protein, mGarnet2, for microscopy and STED nanoscopy.

作者信息

Matela Gabriela, Gao Peng, Guigas Gernot, Eckert Antonia F, Nienhaus Karin, Nienhaus G U

机构信息

Institute of Applied Physics, Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany.

Institute of Applied Physics, Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany and Institute of Nanotechnology and Institute of Toxicology and Genetics, Karlsruhe Institute of Technology (KIT), Eggenstein-Leopoldshafen, Germany.

出版信息

Chem Commun (Camb). 2017 Jan 10;53(5):979-982. doi: 10.1039/c6cc09081h.

DOI:10.1039/c6cc09081h

PMID:28044150

Abstract

Here we present mGarnet2, a monomeric, far-red fluorescent marker protein derived from mRuby, with absorption and emission bands peaking at 598 and 671 nm, respectively. The protein shows excellent performance as a live-cell fusion marker for STED nanoscopy with 640 nm excitation and 780 nm depletion wavelengths.

摘要

在此，我们展示了mGarnet2，一种源自mRuby的单体远红荧光标记蛋白，其吸收峰和发射峰分别位于598纳米和671纳米处。该蛋白作为用于受激发射损耗（STED）纳米显微镜的活细胞融合标记物表现出色，激发波长为640纳米，损耗波长为780纳米。