Mi X Q, Chen J Y, Cen Y, Liang Z J, Zhou L W
Medical School, Fudan University, Shanghai, China.
J Photochem Photobiol B. 2004 Mar 19;74(1):7-12. doi: 10.1016/j.jphotobiol.2004.01.003.
The effects of laser irradiation with 632.8 and 532 nm on rheological properties of blood were comparatively studied in vitro. Under the irradiation condition of 30 mW, laser irradiation of blood samples using a spot diameter of 5 mm with each laser, showed promising results in the modulation of hemorheological properties. When blood samples from patients with abnormally high values of erythrocyte sedimentation rate (ESR) were irradiated, the values of ESR were lowered statistically by either of the 632.8 or 532 nm lasers. The laser irradiation reduced blood viscosities at different shear rates (10-110 S(-1)) for the hyper-viscosity blood samples. Laser irradiation increased the electrophoretic mobility (EPM) of erythrocytes when the values of the sample's EPM were abnormally slow. The erythrocyte deformability was enhanced by laser irradiation when the deformability of the sample from the patients was originally poor. For verifying the improvement of laser irradiation on erythrocyte deformability, the typical erythrocyte samples with poor deformability were produced by the pre-treatment of the erythrocytes with Ca(2+). The deformability of these erythrocyte samples was also improved after laser irradiation. These results suggest that membrane-bound hemoglobin (Hbm) might be the initial site of the interaction, since Hbm is the main cause of poor deformability when erythrocytes were treated with Ca(2+). In all experiments including ESR, blood viscosity, EPM and erythrocyte deformability, the 532 nm laser demonstrated more efficient effects on modulating rheological properties than 632.8 nm laser. This wavelength effect is consistent with the absorption spectrum of hemoglobin, reflecting that hemoglobin may be one of the action targets under laser irradiation.
体外比较研究了632.8 nm和532 nm激光照射对血液流变学特性的影响。在30 mW的照射条件下，使用每种激光以5 mm的光斑直径照射血液样本，在血液流变学特性调节方面显示出有前景的结果。当照射红细胞沉降率（ESR）异常高值患者的血液样本时，632.8 nm或532 nm激光均可使ESR值在统计学上降低。激光照射降低了高粘度血液样本在不同剪切速率（10 - 110 S(-1)）下的血液粘度。当样本的电泳迁移率（EPM）异常缓慢时，激光照射可增加红细胞的电泳迁移率。当患者样本的红细胞变形性原本较差时，激光照射可增强其变形性。为验证激光照射对红细胞变形性的改善作用，通过用Ca(2+)预处理红细胞制备了典型的变形性差的红细胞样本。激光照射后这些红细胞样本的变形性也得到了改善。这些结果表明，膜结合血红蛋白（Hbm）可能是相互作用的初始位点，因为当红细胞用Ca(2+)处理时，Hbm是变形性差的主要原因。在包括ESR、血液粘度、EPM和红细胞变形性的所有实验中，532 nm激光在调节流变学特性方面比632.8 nm激光表现出更有效的效果。这种波长效应与血红蛋白的吸收光谱一致，反映出血红蛋白可能是激光照射下的作用靶点之一。
