Stadler I, Evans R, Kolb B, Naim J O, Narayan V, Buehner N, Lanzafame R J
The Laser Center and Department of Surgery, Rochester General Hospital, Rochester, New York 14621, USA.
Lasers Surg Med. 2000;27(3):255-61. doi: 10.1002/1096-9101(2000)27:3<255::aid-lsm7>3.0.co;2-l.
The effects of low-level laser light irradiation are still highly contested, and the mechanisms of its action still unclear. This study was conducted to test the effects of low-level laser irradiation at 660 nm on human lymphocytes and to investigate the possible mechanisms by which these effects are produced.
STUDY DESIGN/MATERIALS AND METHODS: Whole blood obtained by phlebotomy was irradiated at 660 nm by using energy fluences between 0 and 5.0 J/cm(2). The lymphocytes were isolated after irradiation of the whole blood. For the control experiment, the lymphocytes were first isolated and then irradiated at the same wavelength and energy fluence for comparison. The proliferation of lymphocytes and the formation of free radicals and lipid peroxides were monitored. Hemoglobin was also irradiated in a cell-free environment to test for the production of lipid peroxides.
Lymphocyte proliferation was significantly higher (P<0.05) as expressed by a Stimulation Index in samples irradiated in the presence of whole blood compared with lymphocytes irradiated after isolation from whole blood. Free radical and lipid peroxide production also increased significantly when samples were irradiated in the presence of red blood cells.
The present study supports the hypothesis that one mechanism for the photobiostimulation effect after irradiation at 660 nm is the reaction of light with hemoglobin, resulting in oxygen radical production.
低强度激光照射的效果仍存在高度争议，其作用机制也尚不清楚。本研究旨在测试660nm低强度激光照射对人淋巴细胞的影响，并探究产生这些影响的可能机制。
研究设计/材料与方法：通过静脉穿刺采集的全血，使用0至5.0J/cm(2)的能量通量在660nm下进行照射。全血照射后分离淋巴细胞。作为对照实验，先分离淋巴细胞，然后在相同波长和能量通量下进行照射以作比较。监测淋巴细胞的增殖以及自由基和脂质过氧化物的形成。还在无细胞环境中照射血红蛋白以测试脂质过氧化物的产生。
与从全血中分离后照射的淋巴细胞相比，在全血存在下照射的样本中，以刺激指数表示的淋巴细胞增殖显著更高（P<0.05）。当样本在红细胞存在下照射时，自由基和脂质过氧化物的产生也显著增加。
本研究支持以下假设，即660nm照射后光生物刺激效应的一种机制是光与血红蛋白反应，导致氧自由基产生。
