Lo Cascio Patrizia, Calabrò Concetta, Bertuccio Clara, Paterniti Irene, Palombieri Deborah, Calò Margherita, Albergamo Ambrogina, Salvo Andrea, Gabriella Denaro Maria
a Department of Chemical, Biological, Pharmacological and Environmental Sciences , University of Messina , Messina , Italy.
b Department of Veterinary Sciences , University of Messina , Messina , Italy.
Nat Prod Res. 2017 Jul;31(13):1478-1485. doi: 10.1080/14786419.2016.1274893. Epub 2017 Jan 3.
In the present work, morphological and molecular effects of short-term feed deprivation and refeeding with Spirulina (Arthrospira platensis) on zebrafish digestive tract were determined. Once elucidated the proximate composition of Spirulina feed, immunohistochemical and western blot analyses of peptide transporter (PepT1) and cholecystokinin (CCK8) were carried out in the gastrointestinal tract of zebrafish, previously morphologically investigated. Two and five fasting days caused not only morphostructural alterations, but also the downregulation of PepT1 and CCK8 proteins. Conversely, the recovery of normal morphological conditions, along with an increased PepT1 and CCK8 expression, were observed after refeeding with Spirulina. The increase of PepT1 expression in zebrafish may be responsible for the enhanced CCK8 secretion, so that both proteins may contribute to an improved digestion process during refeeding. These observations could be supported not only by compensatory mechanisms induced by fasting and refeeding but also by an higher protein quality of Spirulina-based diet.
在本研究中，确定了短期禁食以及用螺旋藻（钝顶节旋藻）重新投喂对斑马鱼消化道的形态学和分子效应。一旦阐明了螺旋藻饲料的近似成分，就在先前进行过形态学研究的斑马鱼胃肠道中进行了肽转运体（PepT1）和胆囊收缩素（CCK8）的免疫组织化学和蛋白质印迹分析。禁食两天和五天不仅导致形态结构改变，还导致PepT1和CCK8蛋白的下调。相反，在用螺旋藻重新投喂后，观察到正常形态条件的恢复以及PepT1和CCK8表达的增加。斑马鱼中PepT1表达的增加可能是CCK8分泌增强的原因，因此这两种蛋白质可能有助于在重新投喂期间改善消化过程。这些观察结果不仅可以由禁食和重新投喂诱导的补偿机制来支持，还可以由基于螺旋藻的饮食的更高蛋白质质量来支持。
