Department of Cardiology, People's Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou, China.
Department of Cardiology, The First Affiliated Hospital of Zhejiang University, Zhejiang University, Hangzhou, China.
Sci Rep. 2017 Jan 3;7:39918. doi: 10.1038/srep39918.
The aim of the present study was to investigate the expression of circular RNAs (circRNAs) in the peripheral blood of coronary artery disease (CAD) patients and the potential use of circRNAs as diagnostic biomarkers of CAD. We first analysed peripheral blood circRNAs of 12 CAD patients and 12 control individuals by RNA microarray and found that 22 circRNAs were differentially expressed between these two groups: 12 were upregulated, and 10 were downregulated. Then, we selected 5 circRNAs as candidate biomarkers under stricter screening criteria and verified them in another group of subjects consisting of 30 control individuals and 30 CAD patients with different SYNTAX scores. These 5 circRNAs were all remarkably increased in the CAD group. Hsa_circ_0124644 had the largest area under the curve (AUC). We tested hsa_circ_0124644 in an independent cohort consisting of 115 control individuals and 137 CAD patients. After we included the risk factors for CAD, the AUC slightly increased from 0.769 (95% confidence interval = [0.710-0.827], P < 0.001) to 0.804 ([0.751-0.857], P < 0.001), and when combined with hsa_circ_0098964, the diagnostic value slightly increased. Taken together, our results suggest that hsa_circ_0124644 can be used as a diagnostic biomarker of CAD.
本研究旨在探讨环状 RNA（circRNAs）在冠心病（CAD）患者外周血中的表达及其作为 CAD 诊断生物标志物的潜在用途。我们首先通过 RNA 微阵列分析了 12 例 CAD 患者和 12 例对照个体的外周血 circRNAs，发现这两组之间有 22 个 circRNAs 存在差异表达：12 个上调，10 个下调。然后，我们在另一个由 30 例对照个体和 30 例不同 SYNTAX 评分的 CAD 患者组成的研究对象组中，根据更严格的筛选标准选择了 5 个 circRNAs 作为候选生物标志物，并对其进行了验证。这 5 个 circRNAs 在 CAD 组中均显著增加。Hsa_circ_0124644 的曲线下面积（AUC）最大。我们在由 115 例对照个体和 137 例 CAD 患者组成的独立队列中测试了 hsa_circ_0124644。在纳入 CAD 的危险因素后，AUC 从 0.769（95%置信区间[0.710-0.827]，P<0.001）略微增加至 0.804（[0.751-0.857]，P<0.001），当与 hsa_circ_0098964 联合使用时，诊断价值略有提高。总之，我们的研究结果表明，hsa_circ_0124644 可作为 CAD 的诊断生物标志物。
