酿酒酵母染色质重塑因子Fun30的ATP酶C结构域的晶体结构
Crystal structure of the ATPase-C domain of the chromatin remodeller Fun30 from Saccharomyces cerevisiae.
作者信息
Liu Lan, Jiang Tao
机构信息
National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Datun Road, Chaoyang District, Beijing 100101, People's Republic of China.
出版信息
Acta Crystallogr F Struct Biol Commun. 2017 Jan 1;73(Pt 1):9-15. doi: 10.1107/S2053230X16019269.
Abstract
Fun30 (Function unknown now 30) is a chromatin remodeller belonging to the Snf2 family. It has previously been reported to be a regulator of several cellular activities, including DNA repair, gene silencing and maintenance of chromatin structure. Here, the crystal structure of the Fun30 ATPase-C domain (the C-lobe of the ATPase domain) is reported at 1.95 Å resolution. Although the structure displays overall similarities to those of other Snf2 family members, a new structural module was found to be specific to the Fun30 subfamily. Fun30 ATPase-C was shown be monomeric in solution and showed no detectable affinity for dsDNA.
摘要
Fun30（目前功能未知的30）是一种属于Snf2家族的染色质重塑因子。此前有报道称它是多种细胞活动的调节因子，包括DNA修复、基因沉默和染色质结构维持。在此，报道了Fun30 ATP酶-C结构域（ATP酶结构域的C叶）的晶体结构，分辨率为1.95 Å。尽管该结构与其他Snf2家族成员的结构总体相似，但发现了一个新的结构模块是Fun30亚家族特有的。Fun30 ATP酶-C在溶液中呈单体形式，对双链DNA没有可检测到的亲和力。
相似文献
1
Crystal structure of the ATPase-C domain of the chromatin remodeller Fun30 from Saccharomyces cerevisiae.酿酒酵母染色质重塑因子Fun30的ATP酶C结构域的晶体结构
Acta Crystallogr F Struct Biol Commun. 2017 Jan 1;73(Pt 1):9-15. doi: 10.1107/S2053230X16019269.
2
The Snf2 homolog Fun30 acts as a homodimeric ATP-dependent chromatin-remodeling enzyme.Snf2 同源物 Fun30 作为同源二聚体 ATP 依赖性染色质重塑酶发挥作用。
J Biol Chem. 2010 Mar 26;285(13):9477-9484. doi: 10.1074/jbc.M109.082149. Epub 2010 Jan 14.
3
The SNF2-family member Fun30 promotes gene silencing in heterochromatic loci.SNF2 家族成员 Fun30 促进异染色质位点的基因沉默。
PLoS One. 2009 Dec 1;4(12):e8111. doi: 10.1371/journal.pone.0008111.
4
The Fun30 nucleosome remodeller promotes resection of DNA double-strand break ends.Fun30 核小体重塑酶促进 DNA 双链断裂末端的切除。
Nature. 2012 Sep 27;489(7417):576-80. doi: 10.1038/nature11355. Epub 2012 Sep 9.
5
Mechanism of chromatin remodelling revealed by the Snf2-nucleosome structure.Snf2-核小体结构揭示的染色质重塑机制。
Nature. 2017 Apr 27;544(7651):440-445. doi: 10.1038/nature22036. Epub 2017 Apr 19.
6
The yeast Fun30 and human SMARCAD1 chromatin remodellers promote DNA end resection.酵母 Fun30 和人类 SMARCAD1 染色质重塑因子促进 DNA 末端切除。
Nature. 2012 Sep 27;489(7417):581-4. doi: 10.1038/nature11353. Epub 2012 Sep 9.
7
The Saccharomyces cerevisiae chromatin remodeler Fun30 regulates DNA end resection and checkpoint deactivation.酿酒酵母染色质重塑因子 Fun30 调节 DNA 末端切除和检验点失活。
Mol Cell Biol. 2012 Nov;32(22):4727-40. doi: 10.1128/MCB.00566-12. Epub 2012 Sep 24.
8
A SAM-key domain required for enzymatic activity of the Fun30 nucleosome remodeler.一个 SAM 结构域对于 Fun30 核小体重塑酶的酶活性是必需的。
Life Sci Alliance. 2023 Jul 19;6(9). doi: 10.26508/lsa.202201790. Print 2023 Sep.
9
The ATP-dependent chromatin remodeling enzyme Fun30 represses transcription by sliding promoter-proximal nucleosomes.ATP 依赖的染色质重塑酶 Fun30 通过滑动启动子近端核小体来抑制转录。
J Biol Chem. 2013 Aug 9;288(32):23182-93. doi: 10.1074/jbc.M113.471979. Epub 2013 Jun 18.
10
Mechanism of DNA translocation underlying chromatin remodelling by Snf2.Snf2 介导的染色质重塑过程中 DNA 易位的机制。
Nature. 2019 Mar;567(7748):409-413. doi: 10.1038/s41586-019-1029-2. Epub 2019 Mar 13.
引用本文的文献
1
Fun30 nucleosome remodeller regulates white-to-opaque switching in .Fun30 核小体重塑因子调节. 的白- opaque 转换。
Acta Biochim Biophys Sin (Shanghai). 2023 Mar 25;55(3):508-517. doi: 10.3724/abbs.2023031.
2
Nucleosome Remodeling by Fun30 in the DNA Damage Response.Fun30在DNA损伤应答中的核小体重塑作用
Front Mol Biosci. 2019 Sep 4;6:78. doi: 10.3389/fmolb.2019.00078. eCollection 2019.
本文引用的文献
1
Processing of X-ray diffraction data collected in oscillation mode.振荡模式下收集的X射线衍射数据的处理。
Methods Enzymol. 1997;276:307-26. doi: 10.1016/S0076-6879(97)76066-X.
2
Structure of chromatin remodeler Swi2/Snf2 in the resting state.染色质重塑酶 Swi2/Snf2 在静息状态下的结构。
Nat Struct Mol Biol. 2016 Aug;23(8):722-9. doi: 10.1038/nsmb.3259. Epub 2016 Jul 11.
3
The Fun30 chromatin remodeler Fft3 controls nuclear organization and chromatin structure of insulators and subtelomeres in fission yeast.Fun30染色质重塑因子Fft3控制裂殖酵母中绝缘子和亚端粒的核组织和染色质结构。
PLoS Genet. 2015 Mar 23;11(3):e1005101. doi: 10.1371/journal.pgen.1005101. eCollection 2015 Mar.
4
Deciphering key features in protein structures with the new ENDscript server.利用新的 ENDscript 服务器破译蛋白质结构中的关键特征。
Nucleic Acids Res. 2014 Jul;42(Web Server issue):W320-4. doi: 10.1093/nar/gku316. Epub 2014 Apr 21.
5
The ATP-dependent chromatin remodeling enzyme Fun30 represses transcription by sliding promoter-proximal nucleosomes.ATP 依赖的染色质重塑酶 Fun30 通过滑动启动子近端核小体来抑制转录。
J Biol Chem. 2013 Aug 9;288(32):23182-93. doi: 10.1074/jbc.M113.471979. Epub 2013 Jun 18.
6
SWI/SNF-like chromatin remodeling factor Fun30 supports point centromere function in S. cerevisiae.SWI/SNF 样染色质重塑因子 Fun30 支持酿酒酵母中的点状着丝粒功能。
PLoS Genet. 2012 Sep;8(9):e1002974. doi: 10.1371/journal.pgen.1002974. Epub 2012 Sep 27.
7
The yeast Fun30 and human SMARCAD1 chromatin remodellers promote DNA end resection.酵母 Fun30 和人类 SMARCAD1 染色质重塑因子促进 DNA 末端切除。
Nature. 2012 Sep 27;489(7417):581-4. doi: 10.1038/nature11353. Epub 2012 Sep 9.
8
The Fun30 nucleosome remodeller promotes resection of DNA double-strand break ends.Fun30 核小体重塑酶促进 DNA 双链断裂末端的切除。
Nature. 2012 Sep 27;489(7417):576-80. doi: 10.1038/nature11355. Epub 2012 Sep 9.
9
Towards automated crystallographic structure refinement with phenix.refine.利用phenix.refine实现自动化晶体学结构精修
Acta Crystallogr D Biol Crystallogr. 2012 Apr;68(Pt 4):352-67. doi: 10.1107/S0907444912001308. Epub 2012 Mar 16.
10
Maintenance of silent chromatin through replication requires SWI/SNF-like chromatin remodeler SMARCAD1.通过复制维持沉默染色质需要具有 SWI/SNF 样染色质重塑因子 SMARCAD1。
Mol Cell. 2011 May 6;42(3):285-96. doi: 10.1016/j.molcel.2011.02.036.
Zotero 插件