Snf2 同源物 Fun30 作为同源二聚体 ATP 依赖性染色质重塑酶发挥作用。
The Snf2 homolog Fun30 acts as a homodimeric ATP-dependent chromatin-remodeling enzyme.
机构信息
Department of Biochemistry, Faculty of Medicine and Health Sciences, United Arab Emirates University, P.O. Box 17666, Al-Ain, United Arab Emirates; Wellcome Trust Centre for Gene Regulation and Expression, School of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, United Kingdom.
Wellcome Trust Centre for Gene Regulation and Expression, School of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, United Kingdom.
出版信息
J Biol Chem. 2010 Mar 26;285(13):9477-9484. doi: 10.1074/jbc.M109.082149. Epub 2010 Jan 14.
The Saccharomyces cerevisiae Fun30 (Function unknown now 30) protein shares homology with an extended family of Snf2-related ATPases. Here we report the purification of Fun30 principally as a homodimer with a molecular mass of about 250 kDa. Biochemical characterization of this complex reveals that it has ATPase activity stimulated by both DNA and chromatin. Consistent with this, it also binds to both DNA and chromatin. The Fun30 complex also exhibits activity in ATP-dependent chromatin remodeling assays. Interestingly, its activity in histone dimer exchange is high relative to the ability to reposition nucleosomes. Fun30 also possesses a weakly conserved CUE motif suggesting that it may interact specifically with ubiquitinylated proteins. However, in vitro Fun30 was found to have no specificity in its interaction with ubiquitinylated histones.
酿酒酵母 Fun30（Function unknown now 30）蛋白与一个扩展的 Snf2 相关 ATP 酶家族具有同源性。在这里，我们报告了 Fun30 的主要纯化作为一个同二聚体，分子量约为 250 kDa。该复合物的生化特性表明，它具有由 DNA 和染色质均刺激的 ATP 酶活性。与此一致，它也与 DNA 和染色质结合。Fun30 复合物还在 ATP 依赖性染色质重塑测定中表现出活性。有趣的是，它在组蛋白二聚体交换中的活性相对于核小体重新定位的能力很高。Fun30 还具有一个弱保守的 CUE 基序，表明它可能与泛素化蛋白特异性相互作用。然而，体外 Fun30 与泛素化组蛋白的相互作用没有特异性。
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