Dotti Silvia, Lombardo Tina, Villa Riccardo, Cacciamali Andrea, Zanotti Cinzia, Andreani Nadia Andrea, Cinotti Stefano, Ferrari Maura
Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna, Brescia, Italy.
PLoS One. 2017 Jan 3;12(1):e0169391. doi: 10.1371/journal.pone.0169391. eCollection 2017.
The key role of cell cultures in different scientific fields is worldwide recognized, both as in vitro research models alternative to laboratory animals and substrates for biological production. However, many safety concerns rise from the use of animal/human cell lines that may be tumorigenic, leading to potential adverse contaminations in cell-derived biologicals. In order to evaluate the suitability of 13 different cell lines for Poliovirus vaccine production, safety and quality, in vitro/in vivo tumorigenicity and Poliovirus propagation properties were evaluated. Our results revealed that non-human primate cell lines CYNOM-K1, FRhK-4, 4MBr-5 and 4647 are free of tumorigenic features and represent highly susceptible substrates for attenuated Sabin Poliovirus strains. In particular, FRhK-4 and 4647 cell lines are characterized by a higher in vitro replication, resulting indicated for the use in large-scale production field.
细胞培养在不同科学领域的关键作用在全球范围内得到认可，它既作为替代实验动物的体外研究模型，又作为生物制品生产的底物。然而，使用可能具有致瘤性的动物/人类细胞系引发了许多安全问题，这可能导致细胞衍生生物制品中出现潜在的不良污染。为了评估13种不同细胞系用于脊髓灰质炎疫苗生产的适用性、安全性和质量，对其体外/体内致瘤性和脊髓灰质炎病毒增殖特性进行了评估。我们的结果表明，非人灵长类细胞系CYNOM-K1、FRhK-4、4MBr-5和4647没有致瘤特征，并且是减毒萨宾脊髓灰质炎病毒株的高度敏感底物。特别是，FRhK-4和4647细胞系具有更高的体外复制能力，表明适用于大规模生产领域。
