移植到大鼠脑第三脑室的小鼠新皮质组织中血管的镶嵌重建。

Mosaic reconstruction of blood vessels in mouse neocortical tissue transplanted into the third ventricle of rat brain.

作者信息

Nakano Y, Takei K, Toya S, Tsukada Y, Ghandour S, Kohsaka S

机构信息

Department of Physiology, School of Medicine, Keio University, Tokyo, Japan.

出版信息

Brain Res. 1989 Sep 4;496(1-2):336-40. doi: 10.1016/0006-8993(89)91084-6.

DOI:10.1016/0006-8993(89)91084-6

PMID:2804644

Abstract

Newborn mouse neocortical tissue was transplanted into the third ventricle of rat brain and the reconstruction and the origin of the blood vessels were investigated by using a monoclonal antibody against mouse endothelial surface antigen-1 (MESA-1). It was clearly demonstrated that some of the blood vessels in the graft originated in the donor mouse neocortical tissue. An India ink perfusion experiment revealed that the blood was supplied to the MESA-1-positive blood vessels. Furthermore, electron microscopic immunohistochemical studies demonstrated the existence of a mosaic reconstruction of blood vessels which consisted of mouse- and rat-derived vascular endothelial cells. It was concluded that the blood vessels originating in the donor tissue and those originating in the host tissue inoculate with each other in the grafted tissue.

摘要

将新生小鼠的新皮质组织移植到大鼠脑的第三脑室，通过使用抗小鼠内皮表面抗原-1（MESA-1）的单克隆抗体研究血管的重建和起源。结果清楚地表明，移植组织中的一些血管起源于供体小鼠的新皮质组织。印度墨汁灌注实验表明，血液供应到MESA-1阳性血管。此外，电子显微镜免疫组织化学研究证明存在由小鼠和大鼠来源的血管内皮细胞组成的血管镶嵌重建。得出的结论是，起源于供体组织的血管和起源于宿主组织的血管在移植组织中相互连接。