Inoue H, Kohsaka S, Yoshida K, Otani M, Toya S, Tsukada Y
Neurosci Lett. 1985 Jun 24;57(3):289-94. doi: 10.1016/0304-3940(85)90507-5.
Newborn mouse cerebral cortex was transplanted into the third ventricle of an adult rat brain. After the operation, the immunosuppressant cyclosporin A was administered daily for two weeks, and the grafted tissue was examined immunohistochemically at the 4th week. Neurons, astroglias and oligodendroglias were positively stained by antibodies against neurofilament, glial fibrillary acid protein and 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase), respectively. The CNPase staining indicated that myelination had occurred in the grafted tissue. Thus both neuronal and glial elements in the grafted tissue could survive and mature in the third ventricle of the different species upon administration of cyclosporin A.
将新生小鼠的大脑皮层移植到成年大鼠脑的第三脑室中。术后，每天给予免疫抑制剂环孢素A，持续两周，并在第4周对移植组织进行免疫组织化学检查。神经元、星形胶质细胞和少突胶质细胞分别被抗神经丝、胶质纤维酸性蛋白和2',3'-环核苷酸3'-磷酸二酯酶（CNPase）的抗体阳性染色。CNPase染色表明移植组织中发生了髓鞘形成。因此，在给予环孢素A后，移植组织中的神经元和胶质成分在不同物种的第三脑室中能够存活并成熟。
