Ikemoto S, Kishimoto T, Nishio S, Wada S, Maekawa M
Department of Urology, Osaka City University Medical School, Japan.
Cancer. 1989 Nov 15;64(10):2076-80. doi: 10.1002/1097-0142(19891115)64:10<2076::aid-cncr2820641018>3.0.co;2-x.
Patients with advanced malignant neoplasms have a variety of abnormal monocyte and lymphocyte functions. The authors examined tumor necrosis factor (TNF) and prostaglandin E2 (PGE2) production of monocytes in 48 patients with bladder cancer and 16 control subjects. Monocytes were isolated from peripheral blood mononuclear cells by adherence to plastic tissue. They were cultured with lipopolysaccharide for 24 hours, and the culture supernatant was obtained. The TNF was measured by enzyme immunoassay using anti-recombinant human TNF antibody, and PGE2 was measured by radioimmunoassay. As a result, in high-stage bladder cancer patients, there was a significant inverse correlation between TNF and PGE2 production of monocytes. However, there was no significant correlation in control subjects and low stage patients. Accordingly, some patients with high-stage bladder cancer had higher TNF production but lower PGE2 production of monocytes, and vice versa.
晚期恶性肿瘤患者存在多种单核细胞和淋巴细胞功能异常。作者检测了48例膀胱癌患者和16名对照者单核细胞的肿瘤坏死因子（TNF）和前列腺素E2（PGE2）生成情况。通过贴壁于塑料组织从外周血单个核细胞中分离出单核细胞。将它们与脂多糖培养24小时，然后获得培养上清液。使用抗重组人TNF抗体通过酶免疫测定法测量TNF，通过放射免疫测定法测量PGE2。结果显示，在晚期膀胱癌患者中，单核细胞的TNF和PGE2生成之间存在显著的负相关。然而，在对照者和低分期患者中没有显著相关性。因此，一些晚期膀胱癌患者单核细胞的TNF生成较高但PGE2生成较低，反之亦然。
